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Cystathionase Antibody (2E12-1C10)

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Western Blot: Cystathionase Antibody (2E12-1C10) [H00001491-M02] - CTH monoclonal antibody (M02), clone 2E12-1C10 Analysis of CTH expression in K-562.
Western Blot: Cystathionase Antibody (2E12-1C10) [H00001491-M02] - CTH monoclonal antibody (M02), clone 2E12-1C10 Analysis of CTH expression in K-562.
Sandwich ELISA: Cystathionase Antibody (2E12-1C10) [H00001491-M02] - Detection limit for recombinant GST tagged CTH is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
2E12-1C10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Cystathionase Antibody (2E12-1C10) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
CTH (AAH15807, 1 a.a. ~ 405 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGRSDVVMGLVSVNCESLHNRLRFLQNSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQHELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAELPAIMTHASVLKNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPPSGSHS
Localization
H00001491-M02
Specificity
CTH - cystathionase (cystathionine gamma-lyase)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CTH
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA.
Publications
Read Publications using
H00001491-M02 in the following applications:

  • WB
    3 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22419888). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cystathionase Antibody (2E12-1C10)

  • cystathionase (cystathionine gamma-lyase)
  • cystathionine gamma-lyase
  • cysteine desulfhydrase
  • EC 4.4.1
  • EC 4.4.1.1
  • gamma-cystathionase
  • homoserine deaminase
  • homoserine dehydratase
  • MGC9471

Background

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cystathionase Antibody (H00001491-M02)(9)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(3)
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Mouse
(3)
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Showing Publications 1 - 9 of 9.
Publications using H00001491-M02 Applications Species
Gambari L, Lisignoli G, Gabusi E et al. Distinctive Expression Pattern of Cystathionine-B-Synthase and Cystathionine-gamma -Lyase Identifies Mesenchymal Stromal Cells Transition to Mineralizing Osteoblasts. J Cell Physiol 2017-01-25 [PMID: 28121025]
Grassi F, Malik Tyagi A, Calvert JW et al. Hydrogen Sulfide Is a Novel Regulator of Bone Formation Implicated in the Bone Loss Induced by Estrogen Deficiency. J Bone Miner Res 2015-11-28 [PMID: 26614970]
Jiang H, Hurt KJ, Breen K et al. Sex-specific dysregulation of cysteine oxidation and the methionine and folate cycles in female cystathionine gamma-lyase null mice: a serendipitous model of the methylfolate trap. Biol Open 2015-08-14 [PMID: 26276101] (WB, Mouse) WB Mouse
Panza E, De Cicco P, Armogida C et al. Role of the cystathionine gamma lyase/hydrogen sulfide pathway in human melanoma progression. Pigment Cell Melanoma Res 2015-01-01 [PMID: 25205294]
Qipshidze N, Metreveli N, Mishra PK et al. Hydrogen Sulfide Mitigates Cardiac Remodeling During Myocardial Infarction via Improvement of Angiogenesis Int J Biol Sci 2012-01-01 [PMID: 22419888] (WB, Mouse) WB Mouse
Kundu S, Munjal C, Tyagi N et al. Folic acid improves inner ear vascularization in hyperhomocysteinemic mice. Hearing Research. 2011-12-24 [PMID: 22222235]
Dickhout JG, Carlisle RE, Jerome DE et al. The integrated stress response modulates cellular redox state via induction of cystathionine ?-lyase: cross-talk between the integrated stress response and thiol metabolism. J Biol Chem 2012-01-03 [PMID: 22215680] (WB, Mouse) WB Mouse
Maclean KN, Sikora J, Kozich V et al. A novel transgenic mouse model of CBS deficient homocystinuria does not incur hepatic steatosis or fibrosis and exhibits a hypercoagulative phenotype that is ameliorated by betaine treatment. Mol Genet Metab. 2010-06-23 [PMID: 20638879]
d'Emmanuele di Villa Bianca R, Sorrentino R, Maffia P et al. Hydrogen sulfide as a mediator of human corpus cavernosum smooth-muscle relaxation. PNAS;106(11):4513-4518. 2009-01-01 [PMID: 19255435]

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Bioinformatics

Gene Symbol CTH
Entrez
OMIM
Uniprot