Home » Cystathionase » Cystathionase Antibodies » Cystathionase Antibody (2E12-1C10)
Novus Biologicals products are now on bio-techne.com

Cystathionase Antibody (2E12-1C10)

 (9 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: Cystathionase Antibody (2E12-1C10) [H00001491-M02] - CTH monoclonal antibody (M02), clone 2E12-1C10 Analysis of CTH expression in K-562.
Western Blot: Cystathionase Antibody (2E12-1C10) [H00001491-M02] - CTH monoclonal antibody (M02), clone 2E12-1C10 Analysis of CTH expression in K-562.
Sandwich ELISA: Cystathionase Antibody (2E12-1C10) [H00001491-M02] - Detection limit for recombinant GST tagged CTH is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
2E12-1C10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Cystathionase Antibody (2E12-1C10) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
CTH (AAH15807, 1 a.a. ~ 405 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGRSDVVMGLVSVNCESLHNRLRFLQNSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQHELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAELPAIMTHASVLKNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPPSGSHS
Localization
H00001491-M02
Specificity
CTH - cystathionase (cystathionine gamma-lyase)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CTH
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA.
Publications
Read Publications using
H00001491-M02 in the following applications:

  • WB
    3 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22419888). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cystathionase Antibody (2E12-1C10)

  • cystathionase (cystathionine gamma-lyase)
  • cystathionine gamma-lyase
  • cysteine desulfhydrase
  • EC 4.4.1
  • EC 4.4.1.1
  • gamma-cystathionase
  • homoserine deaminase
  • homoserine dehydratase
  • MGC9471

Background

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CBS Antibody
NBP1-33518
Western Blot CBS Antibody
Immunoprecipitation CBS Antibody
Western Blot CBS Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, KD, KO, WB
MPST Antibody
NBP1-82617
Western Blot MPST Antibody
Immunohistochemistry-Paraffin MPST Antibody
Immunocytochemistry/ Immunofluorescence MPST Antibody
(13 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
Peroxiredoxin 1
Antibody
NBP1-82558
Immunohistochemistry-Paraffin Peroxiredoxin 1 Antibody
Immunohistochemistry-Paraffin Peroxiredoxin 1 Antibody
Immunohistochemistry-Paraffin Peroxiredoxin 1 Antibody
(8 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
PAG1 Antibody
(504004) [Unconjugate
MAB5285
Simple Western PAG1 Antibody (504004) [Unconjugated]
Western Blot PAG1 Antibody (504004) [Unconjugated]
(1 Publication)
Species: Hu
Applications: Simple Western, WB
Glutaminyl-peptide
Cyclotransferase/QPCT ...
NBP1-81838
Western Blot Glutaminyl-peptide Cyclotransferase/QPCT Antibody
Immunohistochemistry Glutaminyl-peptide Cyclotransferase/QPCT Antibody
Immunohistochemistry-Paraffin Glutaminyl-peptide Cyclotransferase/QPCT Antibody
(1 Publication)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
Peroxiredoxin 5
Antibody [Unconjugate
AF5724
Western Blot Peroxiredoxin 5 Antibody [Unconjugated]
Immunocytochemistry Peroxiredoxin 5 Antibody [Unconjugated]
Immunocytochemistry Peroxiredoxin 5 Antibody [Unconjugated]
Species: Hu, Mu, Rt
Applications: ICC, WB
PDXP Antibody -
Azide and BSA Free
NBP2-93574
Western Blot PDXP Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence PDXP Antibody - Azide and BSA Free
Immunohistochemistry-Paraffin PDXP Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Myelin PLP Antibody
NB100-74503
Western Blot Myelin PLP Antibody
Western Blot Myelin PLP Antibody
Western Blot Myelin PLP Antibody
(7 Publications)
Species: Mu, Rt
Applications: ICC/IF, WB
PTHLH/PTHrP
Antibody (677939) [Un ...
MAB6734
Immunohistochemistry PTHLH/PTHrP Antibody (677939) [Unconjugated]
(2 Publications)
Species: Hu
Applications: IHC
Catalase Antibody
[Unconjugated]
AF3398
Western Blot Catalase Antibody [Unconjugated]
Simple Western Catalase Antibody [Unconjugated]
Knockout Validated Catalase Antibody [Unconjugated]
(3 Reviews)
(15 Publications)
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(24 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
SRGN Antibody
NBP1-80886
Western Blot SRGN Antibody
Immunohistochemistry-Paraffin SRGN Antibody
Immunohistochemistry-Paraffin SRGN Antibody
(4 Publications)
Species: Hu
Applications: Flow, IHC, IHC-P, IP, WB
MTHFR Antibody
(5D3) - BSA Free
NBP2-37607
Immunohistochemistry-Paraffin MTHFR Antibody (5D3) - BSA Free
ELISA MTHFR Antibody (5D3) - BSA Free
Western Blot MTHFR Antibody (5D3) - BSA Free
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, WB
GCLC Antibody (3H1)
H00002729-M01
ELISA GCLC Antibody (3H1)
Western Blot GCLC Antibody (3H1)
Immunocytochemistry/ Immunofluorescence GCLC Antibody (3H1)
(17 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
iNOS Antibody -
BSA Free
NB300-605
Western Blot iNOS Antibody - BSA Free
Western Blot iNOS Antibody - BSA Free
Western Blot iNOS Antibody - BSA Free
(3 Reviews)
(130 Publications)
Species: Hu, Mu, Po, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB

Publications for Cystathionase Antibody (H00001491-M02)(9)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(3)
All Applications
Filter By Species
Mouse
(3)
All Species
Showing Publications 1 - 9 of 9.
Publications using H00001491-M02 Applications Species
Gambari L, Lisignoli G, Gabusi E et al. Distinctive Expression Pattern of Cystathionine-B-Synthase and Cystathionine-gamma -Lyase Identifies Mesenchymal Stromal Cells Transition to Mineralizing Osteoblasts. J Cell Physiol 2017-01-25 [PMID: 28121025]
Grassi F, Malik Tyagi A, Calvert JW et al. Hydrogen Sulfide Is a Novel Regulator of Bone Formation Implicated in the Bone Loss Induced by Estrogen Deficiency. J Bone Miner Res 2015-11-28 [PMID: 26614970]
Jiang H, Hurt KJ, Breen K et al. Sex-specific dysregulation of cysteine oxidation and the methionine and folate cycles in female cystathionine gamma-lyase null mice: a serendipitous model of the methylfolate trap. Biol Open 2015-08-14 [PMID: 26276101] (WB, Mouse) WB Mouse
Panza E, De Cicco P, Armogida C et al. Role of the cystathionine gamma lyase/hydrogen sulfide pathway in human melanoma progression. Pigment Cell Melanoma Res 2015-01-01 [PMID: 25205294]
Qipshidze N, Metreveli N, Mishra PK et al. Hydrogen Sulfide Mitigates Cardiac Remodeling During Myocardial Infarction via Improvement of Angiogenesis Int J Biol Sci 2012-01-01 [PMID: 22419888] (WB, Mouse) WB Mouse
Kundu S, Munjal C, Tyagi N et al. Folic acid improves inner ear vascularization in hyperhomocysteinemic mice. Hearing Research. 2011-12-24 [PMID: 22222235]
Dickhout JG, Carlisle RE, Jerome DE et al. The integrated stress response modulates cellular redox state via induction of cystathionine ?-lyase: cross-talk between the integrated stress response and thiol metabolism. J Biol Chem 2012-01-03 [PMID: 22215680] (WB, Mouse) WB Mouse
Maclean KN, Sikora J, Kozich V et al. A novel transgenic mouse model of CBS deficient homocystinuria does not incur hepatic steatosis or fibrosis and exhibits a hypercoagulative phenotype that is ameliorated by betaine treatment. Mol Genet Metab. 2010-06-23 [PMID: 20638879]
d'Emmanuele di Villa Bianca R, Sorrentino R, Maffia P et al. Hydrogen sulfide as a mediator of human corpus cavernosum smooth-muscle relaxation. PNAS;106(11):4513-4518. 2009-01-01 [PMID: 19255435]

Reviews for Cystathionase Antibody (H00001491-M02) (0)

There are no reviews for Cystathionase Antibody (H00001491-M02). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cystathionase Antibody (H00001491-M02) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Cystathionase Products

Research Areas for Cystathionase Antibody (H00001491-M02)

Find related products by research area.

Blogs on Cystathionase

There are no specific blogs for Cystathionase, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Cystathionase Antibody (2E12-1C10) and receive a gift card or discount.


Bioinformatics

Gene Symbol CTH
Entrez
OMIM
Uniprot