![Western Blot: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25429_rabbit-cyp27a1-mab-hl1820-azide-and-bsa-free-2120249374976.jpg "Western Blot: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1820 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of human CYP27A1. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CYP27A1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Immunohistochemistry PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Immunohistochemistry_16650.jpg)
![Western Blot PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Western_Blot_16656.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for CYP27A1 Antibody (NBP3-25429)Find related products by research area.
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| Gene Symbol | CYP27A1 |
![Immunocytochemistry/Immunofluorescence: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - CYP27A1 antibody [HL1820] detects CYP27A1 protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: CYP27A1 stained by CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25429_rabbit-cyp27a1-mab-hl1820-azide-and-bsa-free-2120249374969.jpg "Immunocytochemistry/Immunofluorescence: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - CYP27A1 antibody [HL1820] detects CYP27A1 protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: CYP27A1 stained by CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Paraffin: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - CYP27A1 antibody [HL1820] detects CYP27A1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. CYP27A1 stained by CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25429_rabbit-cyp27a1-mab-hl1820-azide-and-bsa-free-2120249342711.jpg "Immunohistochemistry-Paraffin: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - CYP27A1 antibody [HL1820] detects CYP27A1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. CYP27A1 stained by CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - CYP27A1 antibody [HL1820] detects CYP27A1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CYP27A1 stained by CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25429_rabbit-cyp27a1-mab-hl1820-azide-and-bsa-free-2120249432835.jpg "Immunohistochemistry-Paraffin: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - CYP27A1 antibody [HL1820] detects CYP27A1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CYP27A1 stained by CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25429_rabbit-cyp27a1-mab-hl1820-azide-and-bsa-free-212024940872.jpg "Western Blot: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Western Blot: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25429_rabbit-cyp27a1-mab-hl1820-azide-and-bsa-free-2120249432852.jpg "Western Blot: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry CYP7A1 Antibody (946902) [Unconjugated]](https://images.novusbio.com/images2/CYP7A1_MAB9120_Immunocytochemistry__Immunofluorescence_19938.jpg)
![N/A Osteoprotegerin/TNFRSF11B [Biotin]](https://images.novusbio.com/images2/DATA_Osteoprotegerin_DY805_ELISA_2238.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
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![N/A CCL27/CTACK [HRP]](https://images.novusbio.com/images2/DATA_CCL27_DCC270_ELISA_615.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")