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CXCL8/IL-8 Antibody [Biotin]

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Product Details

Summary
Reactivity PoSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF535
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF535
Carrier Free
AF535
Carrier Free
AF535-SP
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CXCL8/IL-8 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant porcine IL-8/CXCL8
Ala26-Gln104
Accession # CAA43461
Specificity
Detects porcine IL-8/CXCL8 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human (rh) IL-8/CXCL8, rhGCP-2, rhIP-10, recombinant mouse (rm) CRG-2, rmI-TAC, rhMIG and rmMIG is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL8
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Porcine CXCL8/IL-8 Antibody (Catalog # MAB5351)
ELISA Detection: Porcine CXCL8/IL-8 Biotinylated Antibody (Catalog # BAF535)
Standard: Recombinant Porcine CXCL8/IL-8 (Catalog # 535-IN)
Readout System
Publications
Read Publications using
BAF535 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL8/IL-8 Antibody [Biotin]

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • CXCL8SCYB8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • LUCT
  • LYNAP
  • MDNCF
  • MDNCFb-ENAP
  • member 8
  • MONAP
  • MONAPGCP1
  • NAF
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1

Background

Interleukin 8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of
pro‑inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc.

IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro-inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. IL-8 has also been reported to be angiogenic both in vivo and in vitro.

  1. Van Damme, J. et al. (1998) in The Cytokine Handbook, A.W. Thomson ed., Academic Press, New York. p. 271.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL8/IL-8 Antibody (BAF535)(8)

We have publications tested in 2 confirmed species: Human, Porcine.

We have publications tested in 4 applications: ELISA Detection, ELISA Development, ELISA Development (Detection), Luminex Development.


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ELISA Detection
(2)
ELISA Development
(3)
ELISA Development (Detection)
(1)
Luminex Development
(1)
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Human
(1)
Porcine
(6)
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Showing Publications 1 - 8 of 8.
Publications using BAF535 Applications Species
DE Marin, CV Bulgaru, CA Anghel, GC Pistol, MI Dore, ML Palade, I Taranu Grape Seed Waste Counteracts Aflatoxin B1 Toxicity in Piglet Mesenteric Lymph Nodes Toxins, 2020-12-15;12(12):. 2020-12-15 [PMID: 33333857] (ELISA Detection, Porcine) ELISA Detection Porcine
SA Hall, SH Ison, C Owles, J Coe, DA Sandercock, AJ Zanella Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines MethodsX, 2019-05-17;6(0):1218-1227. 2019-05-17 [PMID: 31193967] (ELISA Detection, Porcine) ELISA Detection Porcine
S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets Infect. Immun., 2017-03-23;85(4):. 2017-03-23 [PMID: 28167667] (ELISA Development (Detection), Porcine) ELISA Development (Detection) Porcine
P Maisonnasse, E Bouguyon, G Piton, A Ezquerra, C Urien, C Deloizy et al. The respiratory DC/macrophage network at steady-state and upon influenza infection in the swine biomedical model Mucosal Immunology 2016-07-01 [PMID: 26530136]
Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tonnesen E The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia. Intensive Care Med, 2007-04-25;33(6):1085-93. 2007-04-25 [PMID: 17457574] (ELISA Development, Human) ELISA Development Human
Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology. Cytometry A, 2006-05-01;69(5):391-5. 2006-05-01 [PMID: 16604540] (Luminex Development, Porcine) Luminex Development Porcine
Brix-Christensen V, Andersen SK, Andersen R, Mengel A, Dyhr T, Andersen NT, Schmitz O, Orskov H, Tonnesen E Acute hyperinsulinemia restrains endotoxin-induced systemic inflammatory response: an experimental study in a porcine model. Anesthesiology, 2004-04-01;100(4):861-70. 2004-04-01 [PMID: 15087621] (ELISA Development, Porcine) ELISA Development Porcine
Bensaude E, Turner JL, Wakeley PR, Sweetman DA, Pardieu C, Drew TW, Wileman T, Powell PP Classical swine fever virus induces proinflammatory cytokines and tissue factor expression and inhibits apoptosis and interferon synthesis during the establishment of long-term infection of porcine vascular endothelial cells. J. Gen. Virol., 2004-04-01;85(0):1029-37. 2004-04-01 [PMID: 15039545] (ELISA Development, Porcine) ELISA Development Porcine

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Bioinformatics

Gene Symbol IL8
Uniprot