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CXCL5/ENA-78 Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF254
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF254
Carrier Free
AF254
Carrier Free
AF254-SP
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CXCL5/ENA-78 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human CXCL5/ENA-78
Ala37-Asn114
Accession # P42830
Specificity
Detects human ENA-78 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with rhGCP-2 is observed and less than 0.05% cross-reactivity with rmGCP-2, rhGRO alpha , rrCINC-1, rmKC, rhNAP-2 and rhIL-8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CXCL5
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human CXCL5/ENA-78 Antibody (Catalog # MAB654)
ELISA Detection: Human CXCL5/ENA-78 Biotinylated Antibody (Catalog # BAF254)
Standard: Recombinant Human CXCL5/ENA-78 (Catalog # 254-XB)
Readout System
Publications
Read Publications using
BAF254 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL5/ENA-78 Antibody [Biotin]

  • chemokine (C-C motif) ligand 5
  • C-X-C motif chemokine 5
  • CXCL5
  • CXCL5/ENA-78
  • ENA78
  • ENA-78
  • ENA-78(1-78)
  • Epithelial-derived neutrophil-activating protein 78
  • Neutrophil-activating peptide ENA-78
  • neutrophil-activating protein 78
  • SCYB5ENA78

Background

CXCL5, also known as epithelial cell-derived neutrophil-activating peptide (ENA-78), is an 8 kDa proinflammatory member of the CXC subfamily of chemokines. Its Glu-Leu-Arg (ELR) motif confers angiogenic properties and distinguishes it from ELR-CXC chemokines which are angiostatic (1 - 3). Human CXCL5 shares 57% amino acid (aa) sequence identity with mouse and rat CXCL5. Among other human ELR+ chemokines, it shares 77% aa sequence identity with CXCL6/GCP-2 and 35% - 51% with CXCL1/GRO alpha, CXCL2/GRO beta, CXCL3/GRO gamma, CXCL7/NAP-2, and CXCL8/IL-8. Inflammatory stimulation upregulates CXCL5 production in multiple hematopoietic cell types, fibroblasts, endothelial cells, and vascular smooth muscle cells. In vivo, CXCL5 is elevated at sites of inflammation and pulmonary fibrosis where it promotes neutrophil infiltration and activation as well as angiogenesis (3 - 6). Its upregulation contributes to increased vascularization, tumor growth, and metastasis in many cancers (6 - 9). Full length CXCL5 (78 aa) is trimmed at the N-terminus by cathepsin G and chymotrypsin to ENA-74 (74 aa) and ENA-70 (70 aa), with the shortened forms showing increased potency relative to full length CXCL5 (10, 11). CXCL5 exerts its effects primarily through interactions with CXCR2 (6, 12). It also binds duffy antigen receptor for chemokines (DARC), which can limit CXCR2-mediated responses (13, 14).

  1. Strieter, R.M. et al. (2005) Cytokine Growth Factor Rev. 16:593.
  2. Balestrieri, M.L. et al. (2008) Cardiovasc. Res. 78:250.
  3. Walz, A.. et al. (1991) J. Exp. Med. 174:1355.
  4. Strieter, R.M. et al. (1992) Immunol. Invest. 21:549.
  5. Koch, A.E. et al. (1994) J. Clin. Invest. 94:1012.
  6. Begley, L.A. et al. (2008) Neoplasia 10:244.
  7. Vandercappellen, J. et al. (2008) Cancer Lett. 267:226.
  8. Arenberg, D.A. et al. (1998) J. Clin. Invest. 102:465.
  9. Miyazaki, H. et al. (2006) Cancer Res. 66:4279.
  10. Wuyts, A. et al. (1999) Eur. J. Biochem. 260:421.
  11. Nufer, O. et al. (1999) Biochemistry 38:636.
  12. Ahuja, S.K. and P.M. Murphy (1996) J. Biol. Chem. 271:20545.
  13. Kashiwazaki, M. et al. (2003) Int. Immunol. 15:1219.
  14. Horton, L.W. et al. (2007) Cancer Res. 67:9791.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CXCL5/ENA-78
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CXCL5/ENA-78 Antibody [Biotin]
Species: Hu
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Publications for CXCL5/ENA-78 Antibody (BAF254)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Development.


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Bioinformatics

Gene Symbol CXCL5
Uniprot