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CXCL5/ENA-78 Antibody (33170) [Unconjugated]

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Human peripheral blood mononculear cells (PBMCs) either treated with LPS (filled histogram) or untreated (open histogram) were stained with Mouse Anti-Human CXCL5/ENA-78 Monoclonal Antibody (Catalog # MAB654), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Human peripheral blood mononculear cells (PBMCs) either treated with LPS (filled histogram) or untreated (open histogram) were stained with Mouse Anti-Human CXCL5/ENA-78 Monoclonal Antibody (Catalog # MAB654), followed ...read more
Recombinant Human CXCL5/ENA-78 (254-XB) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications B/N, CyTOF-ready, ELISA(Cap), Flow
Clone
33170
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC654U-100UG
Alexa Fluor 405
IC654V-100UG
Alexa Fluor 488
IC654G-100UG
Alexa Fluor 532
FAB654X
Alexa Fluor 594
IC654T-100UG
Alexa Fluor 647
IC654R-100UG
Alexa Fluor 700
IC654N-100UG
Alexa Fluor 750
IC654S-100UG
Allophycocyanin
FAB654A
Biotin
FAB654B
CoraFluor 1
FAB654CL1
DyLight 350
FAB654D
DyLight 405
FAB654E
DyLight 488
FAB654K
DyLight 550
FAB654L
DyLight 594
FAB654M
DyLight 650
FAB654W
DyLight 680
FAB654Y
DyLight 755
FAB654Z
FITC
FAB654F
HRP
FAB654H
Janelia Fluor 525
FAB654JF525
Janelia Fluor 549
FAB654I
Janelia Fluor 585
FAB654JF585
Janelia Fluor 635
FAB654JF635
Janelia Fluor 646
FAB654J
Janelia Fluor 669
FAB654JF669
PE
FAB654P
PerCP
FAB654C
mFluor Violet 450 SE
FAB654MFV450
mFluor Violet 500 SE
FAB654MFV500
mFluor Violet 610 SE
FAB654MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB654-SP
Carrier Free
MAB654-500
Carrier Free
IC654V-100UG
Carrier Free
IC654U-100UG
Carrier Free
IC654T-100UG
Carrier Free
IC654S-100UG
Carrier Free
IC654R-100UG
Carrier Free
IC654N-100UG
Carrier Free
IC654G-100UG
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CXCL5/ENA-78 Antibody (33170) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CXCL5/ENA-78
Ala37-Asn114
Accession # P42830
Specificity
Detects human CXCL5/ENA-78 in ELISAs. Does not cross-react with recombinant human CXCL1, 2, or 3.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CXCL5
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 0.65-5 ug/mL
Application Notes
ELISA Detection: Human CXCL5/ENA-78 Biotinylated Antibody (Catalog number BAF254)
Standard: Recombinant Human CXCL5/ENA-78 (Catalog number 254-XB)
Publications
Read Publications using
MAB654 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL5/ENA-78 Antibody (33170) [Unconjugated]

  • chemokine (C-C motif) ligand 5
  • C-X-C motif chemokine 5
  • CXCL5
  • CXCL5/ENA-78
  • ENA78
  • ENA-78
  • ENA-78(1-78)
  • Epithelial-derived neutrophil-activating protein 78
  • Neutrophil-activating peptide ENA-78
  • neutrophil-activating protein 78
  • SCYB5ENA78

Background

CXCL5, also known as ENA-78, is a CXC chemokine that plays a role in inflammatory responses and angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CXCL5/ENA-78 Antibody (33170) [Unconjugated]
Species: Hu
Applications: B/N, CyTOF-ready, ELISA(Cap), Flow

Publications for CXCL5/ENA-78 Antibody (MAB654)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ELISA Development, Mass Cytometry.


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ELISA Development
(1)
Mass Cytometry
(1)
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Bioinformatics

Gene Symbol CXCL5
Uniprot