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CXCL10/IP-10/CRG-2 Antibody [Biotin]

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Product Details

Summary
Reactivity CrSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF1117
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF1117
Carrier Free
AF1117
Carrier Free
AF1117-SP
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CXCL10/IP-10/CRG-2 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant cotton rat CXCL10/IP‑10/CRG‑2
Thr27-Val94
Accession # AAL16935
Specificity
Detects cotton rat CXCL10/IP‑10/CRG‑2 in Western blots. In this format, less than 5% cross-reactivity with recombinant human IP-10 and recombinant mouse CRG-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CXCL10
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL10/IP-10/CRG-2 Antibody [Biotin]

  • C7
  • chemokine (C-X-C motif) ligand 10
  • CRG2
  • CRG-2
  • CXCL10
  • gIP-10
  • IFI10
  • INP10
  • IP-10
  • mob-1
  • SCYB10

Background

IP-10, also named mob-1 in rat and crg-2 in mouse, is a member of the non-ELR-motif containing CXC chemokine subfamily and has been designated CXCL10. It is induced in various cell types (including keratinocytes, endothelial cells, fibroblasts, smooth muscle cells, monocytes/macrophages, astrocytes, neutrophils, hepatocytes, T cells, epithelial cells and mesothelial cells) in response to stimulation by type I and/or type II interferon as well as by LPS. IP-10 has been shown to chemoattract activated CD4+ and CD8+ T cells, activated NK cells and smooth muscle cells. It also induces the adhesion of activated T cells to VCAM and ICAM. In addition, IP-10 has been reported to antagonize the angiogenic activity of FGF. IP-10 binds to and activates CXCR3, the chemokine receptor that is shared by MIG/CXCL9 and I-TAC/CXCL11, two additional non-ELR CXC chemokines that are induced by interferon. Cotton rat IP-10 cDNA encodes a 94 amino acid (aa) residue precursor protein with a putative 21 aa signal peptide and a 73 aa mature protein with one potential N-linked glycosylation site. Mature cotton rat IP-10 shares 66%, 64%, and 66% aa sequence identity with rat, mouse and human IP10, respectively (1‑7).

  1. Vanguri, P. and J.M. Farber (1990) J. Biol. Chem. 265:15049.
  2. Liang, P. et al. (1994) Proc. Natl. Acad. Sci. USA. 91:12515.
  3. Proost, P. et al. (1993) J. Immunol. 150:1000.
  4. Blanco, J.C. et al. (2001) GenBank Accession # AAL16959.
  5. Neville, L.F. (1997) Prog. Growth Factor Res. 8:207.
  6. Belperio, J.A. et al. (2000) J. Leukoc. Biol. 68:1.
  7. Soejima, K. and B.J. Rollins (2001) J. Immunol. 167:6576.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CXCL10
Uniprot