![Immunocytochemistry/Immunofluorescence: CXADR Antibody (271) [NBP2-89645] - Staining of Human CXADR in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CXADR monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.](http://images.novusbio.com/fullsize/CXADR-Antibody-271-Immunocytochemistry-Immunofluorescence-NBP2-89645-img0001.jpg "Immunocytochemistry/Immunofluorescence: CXADR Antibody (271) [NBP2-89645] - Staining of Human CXADR in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CXADR monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, ELISA |
| Clone | 271 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was obtained from a rabbit immunized with purified, recombinant Human CXADR (Accession#: NP_001329.1; Met1-Gly237). |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CXADR |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody will detect Human CXADR in ELISA pair set (Catalog: # NBP2-79296). In a sandwich ELISA, it can be used as a detection antibody when paired with (Catalog: # NBP2-89643). |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CXADR Antibody (NBP2-89645)Find related products by research area.
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| Gene Symbol | CXADR |
![Flow Cytometry: CXADR Antibody (271) [NBP2-89645] - Analysis of Human CXADR expression on HT-29 cells. Cells were stained with purified anti-Human CXADR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CXADR-Antibody-271-Flow-Cytometry-NBP2-89645-img0002.jpg "Flow Cytometry: CXADR Antibody (271) [NBP2-89645] - Analysis of Human CXADR expression on HT-29 cells. Cells were stained with purified anti-Human CXADR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
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