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CTDSPL Overexpression Lysate

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Western Blot: CTDSPL Overexpression Lysate (Adult Normal) [NBL1-09569] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CTDSPL.
Western Blot: CTDSPL Overexpression Lysate (Adult Normal) [NBL1-09569] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CTDSPL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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CTDSPL Overexpression Lysate Summary

Description

CTDSPL Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC214440

Accession#: NM_005808

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
CTDSPL

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (cat# NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for CTDSPL Overexpression Lysate

  • C3orf8
  • Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3
  • CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) smallphosphatase-like
  • CTD small phosphatase-like protein
  • CTDSP-like
  • EC 3.1.3
  • HYA22
  • NIF1
  • NIFL
  • NIF-like protein
  • NLI-interacting factor 1
  • Nuclear LIM interactor-interacting factor 1
  • Protein YA22
  • PSR1
  • RB protein serine phosphatase from chromosome 3
  • RBSP3EC 3.1.3.16
  • SCP3chromosome 3 open reading frame 8
  • Small CTD phosphatase 3
  • Small C-terminal domain phosphatase 3
  • YA22

Background

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation (By similarity). Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CTDSPL