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Crossveinless-2/CV-2/BMPER Antibody (355304)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
355304
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

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Crossveinless-2/CV-2/BMPER Antibody (355304) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Crossveinless‑2/CV‑2
Val34-Arg685
Accession # Q8N8U9
Specificity
Detects human Crossveinless‑2/CV‑2 in direct ELISAs and Western blots. In Western blots, 25% cross-reactivity with recombinant mouse CV-2 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
BMPER
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB1956 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Crossveinless-2/CV-2/BMPER Antibody (355304)

  • BMP binding endothelial regulator
  • BMP-binding endothelial regulator precursor protein
  • BMP-binding endothelial regulator protein
  • BMPER
  • Bone morphogenetic protein-binding endothelial cell precursor-derived regulator
  • CRIM3
  • crossveinless 2
  • Crossveinless-2
  • CV2
  • CV-2
  • hCV2
  • KIAA1965
  • Protein crossveinless-2

Background

Crossveinless-2 (CV-2), also known as bone morphogenetic protein-binding endothelial cell precursor-derived regulator (BMPER), is a secreted Chordin-like protein that modulates the BMP signaling pathway (1‑3). Human CV-2 is synthesized as a 685 amino acid (aa) precursor protein with a putative 39 aa signal peptide, five tandem chordin-like cysteine-rich (CR) domains, a partial von Willebrand factor type D domain (vWD), and a carboxyl trypsin inhibitor-like cysteine-rich domain (TIL) (1, 4). Secreted CV-2 is reported to be proteolytically cleaved to generate two fragments that are disulfide-linked (1, 2). The cleavage site of R&D Systems’ recombinant CV-2 is found to be between Asp369 and Pro370 in the GDPH sequence within the vWD domain. This cleavage is likely due to an autocatalytic mechanism triggered by low pH comparable to that of the late secretory pathway (5). The GDPH sequence is conserved in CV-2 from other species. It is also found in multiple proteins that undergo a similar type of cleavage (5). Human CV-2 message is detected in many tissues, with the highest expression detected in adult brain and adult and fetal lung (1). It is also expressed in Flk-1+ endothelial cell precursors and in primary chondrocytes (2). During embryonic development, CV-2 is expressed in regions of high BMP signaling, such as the posterior primitive streak and the ventral tail bud (4). Human CV-2 shares 92% and 34% aa sequence identity with the mouse and Drosophila homologs, respectively (1, 4). Results from biochemical experiments using recombinant CV-2 show that CV-2 directly interacts with BMP-2, -4, and -6 to antagonize BMP signaling, which can regulate a wide range of differentiation processes (1, 2). In contrast, genetic data from Drosophila suggest that CV-2 potentiates BMP-signaling (6). It is possible that like TSG, CV-2 can positively and negatively modulate BMP signal transduction depending on the cell context (7).

  1. Binnerts, M.E. et al. (2004) Biochem Biophys Res Commun. 315:272.  
  2. Moser, M. et al. (2003) Mol Cell Biol. 23:5664. 
  3. Garcia-Abreu, J. et al. (2002) Gene, 287:39. 
  4. Coffinier, C. et al. (2002) Mech Dev. 119:S179.
  5. Lidell, M.E. et al. (2003) J. Biol. Chem. 278:13944.
  6. Conley, C.A. et al. (2000) Development 127:3947.
  7. Kamimura, M. et al. (2004) Developmental Dynamics 230:434.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Crossveinless-2/CV-2/BMPER Antibody (MAB1956)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC, IHC, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(1)
IHC
(1)
Immunoprecipitation
(1)
Western Blot
(5)
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Human
(5)
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Showing Publications 1 - 5 of 5.
Publications using MAB1956 Applications Species
JS Esser, RE Steiner, M Deckler, H Schmitt, B Engert, S Link, A Charlet, C Patterson, C Bode, Q Zhou, M Moser Extracellular bone morphogenetic protein modulator BMPER and twisted gastrulation homolog 1 preserve arterial-venous specification in zebrafish blood vessel development and regulate Notch signaling in endothelial cells FEBS J., 2018-03-09;0(0):. 2018-03-09 [PMID: 29473997] (Western Blot, Human) Western Blot Human
Satomi-Kobayashi S, Kinugasa M, Kobayashi R, Hatakeyama K, Kurogane Y, Ishida T, Emoto N, Asada Y, Takai Y, Hirata K, Rikitake Y Osteoblast-like differentiation of cultured human coronary artery smooth muscle cells by bone morphogenetic protein endothelial cell precursor-derived regulator (BMPER). J Biol Chem, 2012-07-09;287(36):30336-45. 2012-07-09 [PMID: 22778264] (Western Blot, Human) Western Blot Human
Yao Y, Jumabay M, Ly A, Radparvar M, Wang AH, Abdmaulen R, Bostrom KI Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium. Blood, 2012-04-03;119(21):5037-47. 2012-04-03 [PMID: 22474252] (IHC, Immunoprecipitation, Western Blot, Human) IHC, Immunoprecipitation, Western Blot Human
Heinke J, Kerber M, Rahner S Bone morphogenetic protein modulator BMPER is highly expressed in malignant tumors and controls invasive cell behavior. Oncogene, 2011-10-24;31(24):2919-30. 2011-10-24 [PMID: 22020334] (ICC, Western Blot, Human) ICC, Western Blot Human
Helbing T, Volkmar F, Goebel U, Heinke J, Diehl P, Pahl HL, Bode C, Patterson C, Moser M Kruppel-like factor 15 regulates BMPER in endothelial cells. Cardiovasc. Res., 2009-09-17;85(3):551-9. 2009-09-17 [PMID: 19767294] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol BMPER
Uniprot