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Chordin-like 2/CHRDL2 Antibody (328905) [Unconjugated]

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Chordin-like 2/ CHRDL2 was detected in immersion fixed frozen sections of embryonic mouse cartilage (15 d.p.c.) using 25 µg/mL Rat Anti-Mouse Chordin-like 2/CHRDL2 Monoclonal Antibody (Catalog # MAB2520) overnight at 4 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IP
Clone
328905
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

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Chordin-like 2/CHRDL2 Antibody (328905) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Chordin-like 2/CHRDL2
Gln24-Leu426
Accession # Q8VEA6.1
Specificity
Detects mouse Chordin-like 2/CHRDL2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CHRDL2, recombinant mouse (rm) CHRDL1, or rmChordin is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CHRDL2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chordin-like 2/CHRDL2 Antibody (328905) [Unconjugated]

  • BNF-1
  • Chordin like 2
  • Chordin-like 2
  • chordin-like protein 2
  • CHRDL2

Background

Chordin-like 2 (CHL2; also known as breast tumor novel factor 1/BNF-1), is a secreted glycoprotein that has significant homology to chordin and acts as a TGF-beta superfamily antagonist (1‑3). CHL2 is a chordin family member with structural homology to CHL1 (also known as neuralin or ventroptin) (1, 2). Mouse CHL2401 cDNA encodes a 45‑50 kDa, 426 amino acid (aa) residue precursor protein with a putative 25 aa signal peptide and a 401 aa mature segment. The mature segment contains three 63 aa cysteine-rich von Willebrand type C repeats (CRs) that are conserved among chordin family members in the spacing of 10 cysteine residues (1, 2). It also contains two potential N-linked glycosylation sites and one putative NLS that lies just proximal to the third CR repeat.

Mouse CHL2, like human CHL2, appears to undergo extensive alternate splicing. This splicing generates both secreted and intracellular forms of CHL2, and influences the type of TGF-beta superfamily member bound (1, 2). The CRs of chordin, especially CR1 and CR3, have been shown to be the functional domains for BMP binding (4). The CR1 and CR3 of CHL proteins are most similar to CR3 of chordin (1). Mature mouse and human CHL2 share 71% amino acid identity, while mouse CHL1 and -2 share 40% amino acid identity (2). Like chordin, CHL2401 exhibits BMP inhibitory activity by directly interacting with BMP-4 and preventing binding to its receptor (1). However, another CHL2 isoform with an additional exon 9b has been shown to bind and inhibit Activin A activity as well (2). CHL2401 is not abundantly expressed in mouse embryos, but is detected only in the chondrocytes of developing joints and in the connective tissue of reproductive organs (1). Mouse CHL2 acts to reduce the rate of matrix accumulation in mesenchymal cells, acting as a negative regulator of cartilage formation (1). In the adult mouse, CHL2 is again detected only faintly in liver, kidney, skeletal muscle and testis (1). Expression patterns in human tissue blots are distinct from those expressed in mouse (1, 2).

  1. Nakayama, N. et al. (2004) Development 131:229.
  2. Oren, A. et al. (2004) Gene 331:17.
  3. Wu, I. and M.A. Moses (2003) Gene 311:105.
  4. Larrain, J. et al. (2000) Development 127:821.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CHRDL2
Uniprot