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CREB [p Ser133] Transcription Factor Assay Kit (Colorimetric)

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CREB [p Ser133] Assay Kit (Colorimetric) [KA1308] - Assay of nuclear fractions isolated from stimulated (10 uM Forskolin for 30 minutes) and nonstimulated HepG2 cells demonstrating activated CREB (Phospho-Ser133).

Product Details

Summary
Applications Func
Suitable Sample Type
Nuclear extracts

Order Details

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CREB [p Ser133] Transcription Factor Assay Kit (Colorimetric) Summary

Description
CREB1 (phospho S133) Transcription Factor Assay Kit is a colorimetric method for determining CREB1 DNA binding activity.
Modification
p Ser133
Assay Type
Colorimetric
Sample Volume
10 uL/well. All samples should be assayed in duplicate at minimum.
Kit Type
Transcription Factor Assay Kit (Colorimetric)
Gene
CREB1

Applications/Dilutions

Dilutions
  • Functional
Publications
Read Publication using KA1308.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 96-Well Cover Sheets
  2. Polysorbate 20
  3. TF CREB (Phospho-Ser133) 96-Well Strip Plate
  4. TF CREB (Phospho-Ser133) Positive Control
  5. TF CREB (Phospho-Ser133) Primary Antibody
  6. TF CREB (Phospho-Ser133) Specific Competitor dsDNA
  7. Transcription Factor Antibody Binding Buffer (10X)
  8. Transcription Factor Binding Assay Buffer (4X)
  9. Transcription Factor Developing Solution
  10. Transcription Factor Goat Anti-Rabbit HRP Conjugate
  11. Transcription Factor Reagent A
  12. Transcription Factor Stop Solution
  13. Wash Buffer Concentrate (400X) (5 mL)

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CREB [p Ser133] Transcription Factor Assay Kit (Colorimetric)

  • active transcription factor CREB
  • cAMP responsive element binding protein 1
  • cAMP-response element-binding protein-1
  • cAMP-responsive element-binding protein 1
  • CREB
  • CREB1
  • CREB-1
  • cyclic AMP-responsive element-binding protein 1
  • MGC9284
  • transactivator protein

Background

CREB1 (phospho S133) Transcription Factor Assay Kit is a colorimetric method for determining CREB1 DNA binding activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Publications for CREB Kit (KA1308)(1)

Reviews for CREB Kit (KA1308) (0)

There are no reviews for CREB Kit (KA1308). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
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FAQs for CREB Kit (KA1308). (Showing 1 - 1 of 1 FAQ).

  1. May I know which creb1 antibody is good for ChIP? I found a paper using your rabbit polyclonal creb1 antibody for ChIP but they didn't indicate the catalog number.
    • We are not aware of any of our Creb1 antibodies being used in ChIP at this time. If you can provide us with the PMID of the paper you are referring to I can contact the author. Otherwise, you can use our Innovators Reward Program to try an antibody in a novel species and application.

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Research Areas for CREB Kit (KA1308)

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Blogs on CREB.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Exploring the Many Roles of PGC-1 alpha
The peroxisome proliferator-activated receptor gamma, co-activator 1 (PGC-1 alpha or PPARGC1A) gene encodes a 91 kDa nuclear protein that acts as a transcriptional co-activator involved in energy metabolism. Interaction with PPAR gamma allows it to in...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol CREB1