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CPI17 alpha Antibody (SR1255)

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Western Blot: CPI17 alpha Antibody (SR1255) [NBP3-21616] - Western blot analysis using NBP3-21616 in (1) MCF7 cell lysate; (2) 293T cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IP
Clone
SR1255
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CPI17 alpha Antibody (SR1255) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human CPI17 alpha (Uniprot #: Q96A00)
Specificity
Detects endogenous levels of total CPI17 alpha
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PPP1R14A
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 1:30
  • Western Blot 1:500-1:2000
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for CPI17 alpha Antibody (SR1255)

  • 17 kDa PKC-potentiated inhibitory protein of PP1
  • CPI-1717-kDa PKC-potentiated inhibitory protein of PP1
  • CPI1717-KDa protein
  • PKC-potentiated inhibitory protein of PP1
  • PPP1INL
  • Protein kinase C-potentiated inhibitor protein of 17 kDa
  • protein phosphatase 1 regulatory subunit 14A
  • protein phosphatase 1, regulatory (inhibitor) subunit 14A

Background

PKC Potentiated Inhibitor Protein-17 (CPI-17) is a 17 kDa protein (1). Expressed in smooth muscles and neuronal cells, CPI-17 is a myosin phosphatase inhibitor protein. Phosphorylation of CPI-17 at Thr38 enhances its inhibitory effect by 100 fold (2). Once phosphorylated, CPI-17 inhibits PP1c and MLCP holoenzyme activity (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PPP1R14A