![Western Blot: COUP-TF I/NR2F1 Antibody [NBP1-31259] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with COUP TF1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COUP-TF-I-NR2F1-Antibody-Western-Blot-NBP1-31259-img0010.jpg "Western Blot: COUP-TF I/NR2F1 Antibody [NBP1-31259] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with COUP TF1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human COUP-TF I/NR2F1. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Rat (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NR2F1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 46 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Publications using NBP1-31259 | Applications | Species |
|---|---|---|
| Bhaduri A, Andrews MG, Mancia Leon W et al. Cell stress in cortical organoids impairs molecular subtype specification Nature 2020-02-01 [PMID: 31996853] (IHC-Fr, IF/IHC, Human) | IHC-Fr, IF/IHC | Human |
| Ziffra R Characterizing the epigenomic landscape of the developing human cortex at single-cell resolution Thesis 1905-07-13 | ||
| Ziffra RS, Kim CN, Ross JM Et al. Single-cell epigenomics reveals mechanisms of human cortical development Nature 2021-10-01 [PMID: 34616060] (IF/IHC) | IF/IHC |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
IHC-Fr | Mouse | 04/07/2022 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NR2F1 |
![Immunocytochemistry/Immunofluorescence: COUP-TF I/NR2F1 Antibody [NBP1-31259] - Analysis of HeLa, using COUP TF1 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/COUP-TF-I-NR2F1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31259-img0008.jpg "Immunocytochemistry/Immunofluorescence: COUP-TF I/NR2F1 Antibody [NBP1-31259] - Analysis of HeLa, using COUP TF1 antibody at 1:200 dilution.")
![Immunohistochemistry-Frozen: COUP-TF I/NR2F1 Antibody [NBP1-31259] - Analysis of embryonic mouse brain tissue using COUP-TF I/NR2F1 antibody. Primary antibody dilution:1:200. Citrate retrieval done 30um thick sections. Image from verified customer review.](http://images.novusbio.com/fullsize/COUP-TF-I-NR2F1-Antibody-Immunohistochemistry-Frozen-NBP1-31259-img0011.jpg "Immunohistochemistry-Frozen: COUP-TF I/NR2F1 Antibody [NBP1-31259] - Analysis of embryonic mouse brain tissue using COUP-TF I/NR2F1 antibody. Primary antibody dilution:1:200. Citrate retrieval done 30um thick sections. Image from verified customer review.")
![Immunohistochemistry-Paraffin: COUP-TF I/NR2F1 Antibody [NBP1-31259] - Mouse hind brain. COUP TF1 antibody [N1], N-term diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/COUP-TF-I-NR2F1-Antibody-Immunohistochemistry-Paraffin-NBP1-31259-img0007.jpg "Immunohistochemistry-Paraffin: COUP-TF I/NR2F1 Antibody [NBP1-31259] - Mouse hind brain. COUP TF1 antibody [N1], N-term diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot MLX Antibody [Unconjugated]](https://images.novusbio.com/images2/MLX_AF4186_Western_Blot_5416.jpg)
![Immunocytochemistry MLX Antibody [Unconjugated]](https://images.novusbio.com/images2/af4186_human-mlx-affinity-purified-polyclonal-ab-immunocytochemistry-412202015556.jpg)
![N/A PSG1 [HRP]](https://images.novusbio.com/images2/DATA_PSG1_DPSG10_ELISA_797.jpg)
![N/A PSG1 [HRP]](https://images.novusbio.com/images2/PSG-1_DPSG10_ELISA_164.jpg)
![Immunohistochemistry Angiopoietin-like Protein 1/ANGPTL1 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af2204_human-angiopoietin-like-1-affinity-purified-polyclonal-ab-immunohistochemistry-27122022161939.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
