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COPB2 Antibody (M3A5)

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Product Details

Summary
Reactivity Hu, Rt, Av, Bv, Ca, Ch, Ha, Pm, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IP, AP
Clone
M3A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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COPB2 Antibody (M3A5) Summary

Immunogen
Microtubule-associated protein from goose brain.
Localization
The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
Specificity
Recognizes an epitope shared by the beta-COP protein (110kD) found in most tissue culture cells, and a high MW doublet of brain microtubule-associated protein (MAP 2, 270-300 kD).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
COPB2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoaffinity Purification
  • Immunocytochemistry/ Immunofluorescence 1:20-1:50
  • Immunoprecipitation 1:10-1:500
  • Western Blot
Application Notes
AF: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: CHO cells IP: Use at an assay dependant dilution. Stacked Golgi membranes from rat liver extract. Stains a reticular structure in the perinuclear area of non-neuronal cells (the periphery of the Golgi complex and a population of vesicles scattered throughout the cytoplasm.
Publications
Read Publications using
NB600-102 in the following applications:

Reactivity Notes

kangaroo rat, goose

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.9% Sodium Azide
Purity
Unpurified

Alternate Names for COPB2 Antibody (M3A5)

  • beta prime subunit
  • Beta'-coat protein
  • Beta'-COP
  • betaprime-COP
  • coatomer protein complex, subunit beta 2 (beta prime)
  • coatomer subunit beta'
  • p102

Background

May be used for studies on the effects of various agents influencing energy status, on disrupting the Golgi complex, or on altering the activity of G-proteins or small GTP-binding proteins on the cellular localization of beta-COP, and for the immunoaffinity purification of the beta-COP protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for COPB2 Antibody (NB600-102)(9)

We have publications tested in 2 confirmed species: Rat, Bovine.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(2)
All Applications
Filter By Species
Rat
(2)
Bovine
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB600-102 Applications Species
BD Needham, M Funabashi, MD Adame, Z Wang, JC Boktor, J Haney, WL Wu, C Rabut, MS Ladinsky, SJ Hwang, Y Guo, Q Zhu, JA Griffiths, R Knight, PJ Bjorkman, MG Shapiro, DH Geschwind, DP Holschneid, MA Fischbach, SK Mazmanian A gut-derived metabolite alters brain activity and anxiety behaviour in mice Nature, 2022-02-14;602(7898):647-653. 2022-02-14 [PMID: 35165440]
Schultz V, Barrie JA, Donald CL et al. Oligodendrocytes are susceptible to Zika virus infection in a mouse model of perinatal exposure: Implications for CNS complications Glia 2021-08-01 [PMID: 33942402]
Ostrowski PP, Freeman SA, Fairn G, Grinstein S Dynamic Podosome-Like Structures in Nascent Phagosomes Are Coordinated by Phosphoinositides Dev. Cell 2019-06-06 [PMID: 31231039]
NakaYama H, Abe M, Morimoto C et al. Microglia permit climbing fiber elimination by promoting GABAergic inhibition in the developing cerebellum. Nat Commun. 2018-07-19 [PMID: 30026565]
Cianciola NL, Carlin CR. Adenovirus RID-alpha activates an autonomous cholesterol regulatory mechanism that rescues defects linked to Niemann-Pick disease type C. J Cell Biol187(4):537-52. 2009-11-16 [PMID: 19948501]
McGugan, G et al. Characterization of a Rab11 -like GTPase, EhRab11, of Entamoeba histolytica. Molecular Biochemical Parasitology 1: 1-10. 2003-01-01 [PMID: 12850258]
Welter, B et al. Characterization of a Rab7-like GTPase, EhRab7: a marker for the earky stages of endocytosis in Entamoeba histolytica. Molecular Biochemical Parasitology 121:254-264. 2002-01-01 [PMID: 12034459]
Donaldson JG, Lippincott-Schwartz J, Klausner RD. Guanine nucleotides modulate the effects of brefeldin A in semipermeable cells: regulation of the association of a 110-kD peripheral membrane protein with the Golgi apparatus. J Cell Biol112(4):579-88. 1991-02-01 [PMID: 1993732] (ICC/IF, Bovine, Rat) ICC/IF Bovine, Rat
Donaldson JG, Lippincott-Schwartz J, Bloom GS et al. Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action. J Cell Biol111(6 Pt 1):2295-306. 1990-12-01 [PMID: 227706] (ICC/IF, Rat) ICC/IF Rat

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol COPB2
Entrez