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Mouse Complement C4 ELISA Kit (Colorimetric)

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ELISA: Mouse Complement C4 ELISA Kit (Colorimetric) [NBP3-18791] - Samples were spiked with high concentrations of Mouse Complement C4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Mouse Complement C4 ELISA Kit (Colorimetric) [NBP3-18791] - Samples were spiked with high concentrations of Mouse Complement C4 and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Mouse Complement C4 ELISA Kit (Colorimetric) [NBP3-18791] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
15.63-1000 ng/mL
Sensitivity
9.38 ng/mL

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Mouse Complement C4 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse Complement C4 in samples. No significant cross-reactivity or interference between Mouse Complement C4 and analogues was observed.
Standard Curve Range
15.63-1000 ng/mL
Sensitivity
9.38 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 5.77
Intra-Assay
%CV < 5.04
Spike Recovery
84-108%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
C4A

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse Complement C4 ELISA Kit (Colorimetric)

  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
  • C4
  • complement component 4A (Rodgers blood group)
  • RG

Background

C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa, beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600mg/L. It is a protein of the classical complement pathway. When activated by C1s, a peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol C4A