![ELISA: Human Collagen IV ELISA Kit (Colorimetric) [NBP2-75864] - Samples were spiked with high concentrations of Human Collagen IV and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75864_human-collagen-iv-elisa-kit-colorimetric-132202416232394.jpg "ELISA: Human Collagen IV ELISA Kit (Colorimetric) [NBP2-75864] - Samples were spiked with high concentrations of Human Collagen IV and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Serum, plasma and other biological fluids |
| Standard Curve Range | 0.78 - 50 ng/mL |
| Sensitivity | 0.47 ng/mL |
| Specificity | This kit recognizes Human COL4 in samples. No significant cross-reactivity or interference between Human COL4 and analogues was observed. |
| Standard Curve Range | 0.78 - 50 ng/mL |
| Sensitivity | 0.47 ng/mL |
| Assay Type | Sandwich-ELISA |
| Inter-Assay | CV% < 5.19% |
| Intra-Assay | CV% < 5% |
| Spike Recovery | 86-106% |
| Sample Volume | 100 uL |
| Kit Type | ELISA Kit (Colorimetric) |
| Gene | COL4A1 |
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| Publications |
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| Storage | Storage of components varies. See protocol for specific instructions. |
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| Publication using NBP2-75864 | Applications | Species |
|---|---|---|
| Zuo T, Xie Q, Liu J et al. Macrophage-derived cathepsin S remodels the extracellular matrix to promote liver fibrogenesis Gastroenterology 2023-05-30 [PMID: 37263311] (ELISA) | ELISA |
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| Gene Symbol | COL4A1 |
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![Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - Analysis of Collagen IV in various paraffin embedded human tissues. Image courtesy of product review submitted by Daniel Pirici.](https://images.novusbio.com/images/Collagen-IV-Antibody-Immunohistochemistry-Paraffin-NB120-6586-img0006.jpg "Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - Analysis of Collagen IV in various paraffin embedded human tissues. Image courtesy of product review submitted by Daniel Pirici.")