Mouse Collagen II ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Mouse COL2 in samples. No significant cross-reactivity or interference between Mouse COL2 and analogues was observed. |
Standard Curve Range |
62.50 - 4000 pg/mL |
Sensitivity |
37.50 pg/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
CV% < 5.57% |
Intra-Assay |
CV% < 5.53% |
Spike Recovery |
84-111% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
COL2A1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Mouse Collagen II ELISA Kit (Colorimetric)
Background
The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. In cartilage, Collagen Type II constitutes the bulk of the fibril. Sensitization with Collagen Type II induces an erosive polyarthritis in rats, mice and higher primates which can resemble rheumatoid arthritis and relapsing polychrondritis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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