![Western Blot: CNPase Antibody [NBP2-50058] - Analysis of different tissue lysates using rabbit pAb to CNP, NBP2-50058, dilution 1:5,000 in red: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. Double bands at 46, 48kDa mark correspond to isotypes of the CNP protein. The blot was simultaneously probed with mouse mAb to calretinin, dilution 1:2,000, in green. The single band at 29kDa corresponds to the calretinin protein.](http://images.novusbio.com/fullsize/CNPase-Antibody-Western-Blot-NBP2-50058-img0004.jpg "Western Blot: CNPase Antibody [NBP2-50058] - Analysis of different tissue lysates using rabbit pAb to CNP, NBP2-50058, dilution 1:5,000 in red: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. Double bands at 46, 48kDa mark correspond to isotypes of the CNP protein. The blot was simultaneously probed with mouse mAb to calretinin, dilution 1:2,000, in green. The single band at 29kDa corresponds to the calretinin protein.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Full length human CNPase recombinant protein. [UniProt# P09543] |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CNP |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In western blot, double bands may be detected at 46 and 48kDa, corresponding to the two isotypes of the CNP protein. |
| Theoretical MW | 48 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Supplied as serum |
| Preservative | 0.035% Sodium Azide |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for CNPase Antibody (NBP2-50058)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: CNPase Antibody [NBP2-50058] - Mixed neuron-glial cell cultures stained with NBP2-50058 (green) and monoclonal antibody against GFAP (red, NBP1-05197). The CNPase antibody stains strongly in oligodendrocytes, whereas GFAP labels the intermediate filament in astrocytes. Blue is DNA staining showing the nuclei of these and numerous other cells, most of which are neurons and which do not express either CNPase or GFAP.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50058-img0003.jpg "Immunocytochemistry/Immunofluorescence: CNPase Antibody [NBP2-50058] - Mixed neuron-glial cell cultures stained with NBP2-50058 (green) and monoclonal antibody against GFAP (red, NBP1-05197). The CNPase antibody stains strongly in oligodendrocytes, whereas GFAP labels the intermediate filament in astrocytes. Blue is DNA staining showing the nuclei of these and numerous other cells, most of which are neurons and which do not express either CNPase or GFAP.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")