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Clusterin/APOJ Antibody (Hs-3) [Biotin]

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Product Details

Summary
Reactivity Hu, Bv(-), Ca(-), Fe(-), Po(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
Hs-3
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB500-552AF350
Alexa Fluor 405
NB500-552AF405
Alexa Fluor 488
NB500-552AF488
Alexa Fluor 532
NB500-552AF532
Alexa Fluor 594
NB500-552AF594
Alexa Fluor 647
NB500-552AF647
Alexa Fluor 700
NB500-552AF700
Alexa Fluor 750
NB500-552AF750
CoraFluor 1
NB500-552CL1
DyLight 350
NB500-552UV
DyLight 405
NB500-552V
DyLight 488
NB500-552G
DyLight 550
NB500-552R
DyLight 594
NB500-552DL594
DyLight 650
NB500-552C
DyLight 680
NB500-552FR
DyLight 755
NB500-552IR
FITC
NB500-552F
HRP
NB500-552H
Janelia Fluor 525
NB500-552JF525
Janelia Fluor 549
NB500-552JF549
Janelia Fluor 585
NB500-552JF585
Janelia Fluor 635
NB500-552JF635
Janelia Fluor 646
NB500-552JF646
Janelia Fluor 669
NB500-552JF669
Unconjugated
NB500-552-0.1mg
mFluor Violet 450 SE
NB500-552MFV450
mFluor Violet 500 SE
NB500-552MFV500
mFluor Violet 610 SE
NB500-552MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB500-552-0.1mg
BSA Free
NB500-552-0.1mg
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Clusterin/APOJ Antibody (Hs-3) [Biotin] Summary

Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Specificity
Apolipoprotein J (Hs-3)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CLU
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for Clusterin/APOJ Antibody (Hs-3) [Biotin]

  • 40
  • 40, sulfated glycoprotein 2
  • Aging-associated gene 4 protein
  • aging-associated protein 4
  • APOJ
  • apo-J
  • Apolipoprotein J
  • CLI
  • CLIclusterin (complement lysis inhibitor, SP-40
  • CLU
  • Clusterin
  • Complement cytolysis inhibitor
  • complement lysis inhibitor
  • Complement-associated protein SP-40
  • Ku70-binding protein 1
  • KUB1SGP2
  • MGC24903
  • NA1/NA2
  • SGP-2
  • SP-40
  • sulfated glycoprotein 2
  • Testosterone-repressed prostate message 2
  • testosterone-repressed prostate message 2, apolipoprotein J)
  • TRPM-2
  • TRPM-2TRPM2

Background

Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP-40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid poly-peptide that is posttranslationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, alpha and beta, are associated through disulfide bonds. The beta subunit (also called ApoJalpha) corresponds to residues 23-227. The alpha subunit (also called ApoJbeta) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences beta-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer's disease pathogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CLU