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CLPS Antibody

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Western Blot: CLPS Antibody [NBP1-86109] - Analysis in control (vector only transfected HEK293T lysate) and CLPS over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Orthogonal Strategies: Immunohistochemistry-Paraffin: CLPS Antibody [NBP1-86109] - Staining in human pancreas and liver tissues using anti-CLPS antibody. Corresponding CLPS RNA-seq data are presented for the same ...read more
Immunohistochemistry-Paraffin: CLPS Antibody [NBP1-86109] - Staining of human kidney shows strong positivity in glomeruli.
Immunohistochemistry-Paraffin: CLPS Antibody [NBP1-86109] - Staining of human liver shows low expression as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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CLPS Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NLENGELCMNSAQCKSNCCQHSSALGLARCTSMASENSECSVKTLYGIYYKCPCERGLTCEGDKTIVGSITNTNFGICHDAGR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CLPS
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
CLPS Protein (NBP1-86109PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CLPS Antibody

  • colipase
  • colipase, pancreatic
  • pancreatic colipase preproprotein

Background

The protein encoded by the CLPS gene is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilizing an active conformation and considerably increasing the overall hydrophobic binding site. The gene product allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. This cofactor is only expressed in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CLPS