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Claudin-4 Antibody (382316) [Unconjugated]

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Claudin-4 was detected in immersion fixed PC-3 human prostate cancer cell line (positive staining) and HLDM-2 human Hodgkin's lymphoma cell line (negative staining) using Mouse Anti-Human Claudin-4 Monoclonal Antibody (Catalog # MAB42191) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Claudin-4 was detected in immersion fixed PC-3 human prostate cancer cell line (positive staining) and HLDM-2 human Hodgkin's lymphoma cell line (negative staining) using Mouse Anti-Human Claudin-4 Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
382316
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB42191-SP
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Claudin-4 Antibody (382316) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human Claudin-4
Met1-Val209
Accession # NP_001296
Specificity
Detects human Claudin-4 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Claudin-4 Antibody (382316) [Unconjugated]

  • claudin 4
  • Claudin4
  • Claudin-4
  • CLDN4
  • Clostridium perfringens enterotoxin receptor 1
  • Clostridium perfringens enterotoxin receptor
  • CPE-R
  • CPE-RCPETR
  • CPE-receptor
  • CPETR1
  • hCPE-R
  • WBSCR8
  • WBSCR8CPERCPETR1claudin-4
  • Williams-Beuren syndrome chromosomal region 8 protein

Background

Claudin-4 is a multipass membrane protein in the claudin family of tight junction proteins. Claudin-4 is a 209 amino acid molecule with four transmembrane segments. It is preferentially expressed in intestinal mucosa epithelium and is involved in epithelial tissue development. It is also up‑regulated in several carcinomas. Phosphorylation of Claudin-4 regulates paracellular epithelial permeability. Human Claudin-4 shares 83% aa sequence identity with mouse and rat Claudin-4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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