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Human Claudin-14 ELISA Kit (Colorimetric)

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ELISA: Human Claudin-14 ELISA Kit (Colorimetric) [NBP2-75327] - Samples were spiked with high concentrations of Human Claudin-14 and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Human Claudin-14 ELISA Kit (Colorimetric) [NBP2-75327] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

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Human Claudin-14 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CLDN14 in samples. No significant cross-reactivity or interference between Human CLDN14 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.32%
Intra-Assay
CV% < 5.2%
Spike Recovery
86-110%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CLDN14

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Claudin-14 ELISA Kit (Colorimetric)

  • claudin 14
  • Claudin14
  • Claudin-14
  • CLDN14
  • DFNB29
  • human CLDN14 gene10claudin-14

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. The encoded protein also binds specifically to the WW domain of Yes-associated protein. Defects in this gene are the cause of an autosomal recessive form of nonsyndromic sensorineural deafness. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CLDN14