![Western Blot: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01] - Analysis of GJB3 expression in transfected 293T cell line by GJB3 monoclonal antibody (M01), clone 3B4-1B3.Lane 1: GJB3 transfected lysate(31 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Connexin-31-GJB3-Antibody-3B4-1B3-Western-Blot-H00002707-M01-img0013.jpg "Western Blot: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01] - Analysis of GJB3 expression in transfected 293T cell line by GJB3 monoclonal antibody (M01), clone 3B4-1B3.Lane 1: GJB3 transfected lysate(31 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 3B4-1B3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | GJB3 (AAH12918.1, 1 a.a. ~ 270 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDWKTLQALLSGVNKYSTAFGRIWLSVVFVFRVLVYVVAAERVWGDEQKDFDCNTKQPGCTNVCYDNYFPISNIRLWALQLIFVTCPSLLVILHVAYREERERRHRQKHGDQCAKLYDNAGKKHGGLWWTYLFSLIFKLIIEFLFLYLLHTLWHGFNMPRLVQCANVAPCPNIVDCYIARPTEKKIFTYFMVGASAVCIVLTICELCYLICHRVLRGLHKDKPRGGCSPSSSASRASTCRCHHKLVEAGEVDPDPGNNKLQASAPNLTPI |
| Specificity | GJB3 - gap junction protein, beta 3, 31kDa (connexin 31) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GJB3 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IF and ELISA. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
![Immunohistochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg)
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_16572.jpg)
![Cochlin [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Cochlin [Unconjugated]")
| Publication using H00002707-M01 | Applications | Species |
|---|---|---|
| Tattersall D, Scott CA, Gray C et al. EKV mutant connexin 31 associated cell death is mediated by ER stress. Hum Mol Genet 18(24):4734-45. 2009-12-15 [PMID: 19755382] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Connexin 31/GJB3 Antibody (H00002707-M01)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01] - Analysis of monoclonal antibody to GJB3 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Connexin-31-GJB3-Antibody-3B4-1B3-Immunocytochemistry-Immunofluorescence-H00002707-M01-img0010.jpg "Immunocytochemistry/Immunofluorescence: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01] - Analysis of monoclonal antibody to GJB3 on HeLa cell. Antibody concentration 10 ug/ml.")
![Western Blot: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01] - GJB3 monoclonal antibody (M01), clone 3B4-1B3 Analysis of GJB3 expression in HeLa.](http://images.novusbio.com/fullsize/Connexin-31-GJB3-Antibody-3B4-1B3-Western-Blot-H00002707-M01-img0012.jpg "Western Blot: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01] - GJB3 monoclonal antibody (M01), clone 3B4-1B3 Analysis of GJB3 expression in HeLa.")
![Sandwich ELISA: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01] - Detection limit for recombinant GST tagged GJB3 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Connexin-31-GJB3-Antibody-3B4-1B3-Sandwich-ELISA-H00002707-M01-img0011.jpg "Sandwich ELISA: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01] - Detection limit for recombinant GST tagged GJB3 is approximately 0.3ng/ml as a capture antibody.")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")