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Clathrin Heavy Chain 1/CHC17 Antibody (X22) [DyLight 488]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ha, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
X22
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB300-613AF350
Alexa Fluor 405
NB300-613AF405
Alexa Fluor 488
NB300-613AF488
Alexa Fluor 532
NB300-613AF532
Alexa Fluor 594
NB300-613AF594
Alexa Fluor 647
NB300-613AF647
Alexa Fluor 700
NB300-613AF700
Alexa Fluor 750
NB300-613AF750
Allophycocyanin
NB300-613APC
Biotin
NB300-613B
CoraFluor 1
NB300-613CL1
DyLight 350
NB300-613UV
DyLight 405
NB300-613V
DyLight 550
NB300-613R
DyLight 594
NB300-613DL594
DyLight 650
NB300-613C
DyLight 680
NB300-613FR
DyLight 755
NB300-613IR
FITC
NB300-613F
HRP
NB300-613H
Janelia Fluor 525
NB300-613JF525
Janelia Fluor 549
NB300-613JF549
Janelia Fluor 646
NB300-613JF646
Janelia Fluor 669
NB300-613JF669
PE
NB300-613PE
PerCP
NB300-613PCP
Unconjugated
NB300-613
mFluor Violet 450 SE
NB300-613MFV450
mFluor Violet 500 SE
NB300-613MFV500
mFluor Violet 610 SE
NB300-613MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB300-613
BSA Free
NB300-613
BSA Free
NB300-613-0.025ml
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Clathrin Heavy Chain 1/CHC17 Antibody (X22) [DyLight 488] Summary

Immunogen
Purified human brain clathrin heavy chain.
Epitope
Electron microscopy and proteolysis mapping demonstrate that binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.
Localization
Cytoplasmic
Marker
Clathrin-coated Vesicle Marker
Specificity
Detects clathrin heavy chain.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CLTC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunomicroscopy
  • Immunoprecipitation
  • Western Blot

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 15689492). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Clathrin Heavy Chain 1/CHC17 Antibody (X22) [DyLight 488]

  • CHC
  • CHC17
  • Clathrin Heavy Chain 1
  • Clathrin heavy chain on chromosome 17
  • clathrin, heavy chain (Hc)
  • clathrin, heavy chain
  • clathrin, heavy polypeptide (Hc)
  • clathrin, heavy polypeptide-like 2
  • CLH17
  • CLH-17
  • CLTC
  • CLTCL2
  • Hc
  • KIAA0034

Background

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains (192 kDa) and three light chains (LCa or LCb, 25-29 kDa) and self assembles into a polyhedral cytoskeletal element. Self assembly facilitates clathrin's central role in membrane sorting and selective transport within the cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CLTC
Entrez
Uniprot