![Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) [NBP2-34240] - Paraffin-embedded human stomach tissue sections. pH 9 antigen retrieval. Image from verified customer review. Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/Chromogranin-A-Antibody-LK2H10-+-PHE5-+-CGA-414-Immunohistochemistry-Paraffin-NBP2-34240-img0003.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) [NBP2-34240] - Paraffin-embedded human stomach tissue sections. pH 9 antigen retrieval. Image from verified customer review. Image using the Azide and BSA Free format of this antibody.")
| Reactivity | Hu, Mu, Rt, Po, PmSpecies Glossary |
| Applications | IHC |
| Clone | LK2H10 + PHE5 + CGA/414 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34674) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Human pheochromocytoma (LK2H10 & PHE5) & recombinant human chromogranin A protein (CGA414) (Uniprot: P10645) |
| Localization | Finely granular cytoplasmic |
| Marker | Neuroendocrine Marker |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CHGA |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Publications |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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