Home » Chordin-like 2/CHRDL2 » Chordin-like 2/CHRDL2 Antibodies » Chordin-like 2/CHRDL2 Antibody (272905) [Unconjugated]
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Chordin-like 2/CHRDL2 Antibody (272905) [Unconjugated]

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MCF-7 cells were stained with Mouse Anti-Human Chordin‑like 2/CHRDL2 Monoclonal Antibody (Catalog # MAB24481, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
MCF-7 cells were stained with Mouse Anti-Human Chordin‑like 2/CHRDL2 Monoclonal Antibody (Catalog # MAB24481, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clone
272905
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC24481U-100UG
Alexa Fluor 405
IC24481V-100UG
Alexa Fluor 488
IC24481G-100UG
Alexa Fluor 532
FAB24481X
Alexa Fluor 594
IC24481T-100UG
Alexa Fluor 647
IC24481R-100UG
Alexa Fluor 700
IC24481N-100UG
Alexa Fluor 750
IC24481S-100UG
Allophycocyanin
FAB24481A
Biotin
FAB24481B
CoraFluor 1
FAB24481CL1
DyLight 350
FAB24481D
DyLight 405
FAB24481E
DyLight 488
FAB24481K
DyLight 550
FAB24481L
DyLight 594
FAB24481M
DyLight 650
FAB24481W
DyLight 680
FAB24481Y
DyLight 755
FAB24481Z
FITC
FAB24481F
HRP
FAB24481H
Janelia Fluor 525
FAB24481JF525
Janelia Fluor 549
FAB24481I
Janelia Fluor 585
FAB24481JF585
Janelia Fluor 635
FAB24481JF635
Janelia Fluor 646
FAB24481J
Janelia Fluor 669
FAB24481JF669
PE
FAB24481P
PerCP
FAB24481C
mFluor Violet 450 SE
FAB24481MFV450
mFluor Violet 500 SE
FAB24481MFV500
mFluor Violet 610 SE
FAB24481MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB24481-SP
Carrier Free
IC24481G-100UG
Carrier Free
IC24481V-100UG
Carrier Free
IC24481U-100UG
Carrier Free
IC24481T-100UG
Carrier Free
IC24481S-100UG
Carrier Free
IC24481R-100UG
Carrier Free
IC24481N-100UG
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Chordin-like 2/CHRDL2 Antibody (272905) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Chordin-like 2/CHRDL2
Arg28-Thr451
Accession # NP_056239
Specificity
Detects human Chordin-like 2/CHRDL2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse CHRDL1 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CHRDL2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chordin-like 2/CHRDL2 Antibody (272905) [Unconjugated]

  • BNF-1
  • Chordin like 2
  • Chordin-like 2
  • chordin-like protein 2
  • CHRDL2

Background

CHRDL2, also known as breast tumor novel factor 1 (BNF-1), is a secreted BMP antagonist implicated in chondrocyte development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHRDL2
Uniprot