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Chk1 [p Ser317] Antibody [Unconjugated]

 (10 Publications)
 
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or exposed (+) to 50 J/m2UV-C for the indicated time. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-Chk1 (S317) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2054) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk1 (S317) was detected at approximately 56 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or exposed (+) to 50 J/m2UV-C for the indicated time. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 50 J/m2 ultraviolet light (UV) for 2 hours, loaded at 0.2 mg/mL. A specific band was ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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AF2054-SP
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Chk1 [p Ser317] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide containing human Chk1 S317 site
Modification
p Ser317
Specificity
Detects human, mouse, and rat Chk1 when phosphorylated at S317 in Western blots. Does not recognize Chk1 when unphosphorylated at S317.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHEK1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF2054 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chk1 [p Ser317] Antibody [Unconjugated]

  • Checkpoint, S. pombe, homolog of, 1
  • CHEK1
  • CHK1 (checkpoint, S.pombe) homolog
  • CHK1 checkpoint homolog (S. pombe)
  • Chk1
  • CHK1serine/threonine-protein kinase Chk1
  • EC 2.7.11
  • EC 2.7.11.1

Background

The Chk1 checkpoint kinase is an integral member of a signaling cascade that controls cell cycle progression. In response to genotoxic or replicative stress, Chk1 is phosphorylated by ATM or ATM-related kinases (ATR) at S317. In turn, Chk1 phosphorylates downstream effectors, such as p53 or the Cdc25 phosphatases to halt cell cycle progression and allow time for repair of incurred damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Chk1 Antibody (AF2054)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(7)
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Human
(7)
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Showing Publications 1 - 10 of 10.
Publications using AF2054 Applications Species
Boichuk S, Bikinieva F, Valeeva E et al. Establishment and Characterization of Multi-Drug Resistant p53-Negative Osteosarcoma SaOS-2 Subline Diagnostics (Basel) 2023-08-11 [PMID: 37627905]
Y Lai, M Zhu, W Wu, N Rokutanda, Y Togashi, W Liang, T Ohta HERC2 regulates RPA2 by mediating ATR-induced Ser33 phosphorylation and ubiquitin-dependent degradation Sci Rep, 2019-10-03;9(1):14257. 2019-10-03 [PMID: 31582797] (Western Blot, Human) Western Blot Human
Devakumar Sundaravinayagam, Hye Rim Kim, TingTing Wu, Hyun Hee Kim, Hyun-Seo Lee, Semo Jun et al. miR146a-mediated targeting of FANCM during inflammation compromises genome integrity Oncotarget 2016-07-19 [PMID: 27351285]
Bing Li, Zeng-Fu Shang, Jiao-Jiao Yin, Qin-Zhi Xu, Xiao-Dan Liu, Yu Wang et al. PIG3 Functions in DNA Damage Response through Regulating DNA-PKcs Homeostasis International Journal of Biological Sciences 2013-05-03 [PMID: 23678292]
Lee AY, Chiba T, Truong LN Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint. J. Biol. Chem., 2011-11-28;287(4):2531-43. 2011-11-28 [PMID: 22123827] (Western Blot, Human) Western Blot Human
Boichuk S, Hu L, Makielski K, Pandolfi PP, Gjoerup OV Functional connection between Rad51 and PML in homology-directed repair. PLoS ONE, 2011-10-05;6(10):e25814. 2011-10-05 [PMID: 21998700] (Western Blot, Human) Western Blot Human
Huehls AM, Wagner JM, Huntoon CJ, Geng L, Erlichman C, Patel AG, Kaufmann SH, Karnitz LM Poly(ADP-Ribose) polymerase inhibition synergizes with 5-fluorodeoxyuridine but not 5-fluorouracil in ovarian cancer cells. Cancer Res., 2011-05-25;71(14):4944-54. 2011-05-25 [PMID: 21613406] (Western Blot, Human) Western Blot Human
Boichuk S, Hu L, Hein J Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen. J. Virol., 2010-06-02;84(16):8007-20. 2010-06-02 [PMID: 20519379] (Western Blot, Human) Western Blot Human
Hein J, Boichuk S, Wu J, Cheng Y, Freire R, Jat PS, Roberts TM, Gjoerup OV Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding. J. Virol., 2008-10-15;83(1):117-27. 2008-10-15 [PMID: 18922873] (Western Blot, Human) Western Blot Human
Sakasai R, Tibbetts R RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage. J. Biol. Chem., 2008-03-12;283(20):13549-55. 2008-03-12 [PMID: 18337245] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol CHEK1