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Chk1 Antibody (2G1D5) [FITC]

 
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clone
2G1D5
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-1768AF350
Alexa Fluor 405
NB100-1768AF405
Alexa Fluor 488
NB100-1768AF488
Alexa Fluor 532
NB100-1768AF532
Alexa Fluor 594
NB100-1768AF594
Alexa Fluor 647
NB100-1768AF647
Alexa Fluor 700
NB100-1768AF700
Alexa Fluor 750
NB100-1768AF750
Allophycocyanin
NB100-1768APC
Biotin
NB100-1768B
CoraFluor 1
NB100-1768CL1
DyLight 350
NB100-1768UV
DyLight 405
NB100-1768V
DyLight 488
NB100-1768G
DyLight 550
NB100-1768R
DyLight 594
NB100-1768DL594
DyLight 650
NB100-1768C
DyLight 680
NB100-1768FR
DyLight 755
NB100-1768IR
HRP
NB100-1768H
Janelia Fluor 525
NB100-1768JF525
Janelia Fluor 549
NB100-1768JF549
Janelia Fluor 646
NB100-1768JF646
Janelia Fluor 669
NB100-1768JF669
PE
NB100-1768PE
PerCP
NB100-1768PCP
Unconjugated
NB100-1768
mFluor Violet 450 SE
NB100-1768MFV450
mFluor Violet 500 SE
NB100-1768MFV500
mFluor Violet 610 SE
NB100-1768MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-1768
BSA Free
NB100-1768
BSA Free
NB100-1768SS
Azide and BSA Free
NBP2-80668
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Chk1 Antibody (2G1D5) [FITC] Summary

Immunogen
Purified recombinant fragment of Chk1 expressed in E. coli. [UniProt# O14757]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CHEK1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for Chk1 Antibody (2G1D5) [FITC]

  • Checkpoint, S. pombe, homolog of, 1
  • CHEK1
  • CHK1 (checkpoint, S.pombe) homolog
  • CHK1 checkpoint homolog (S. pombe)
  • Chk1
  • CHK1serine/threonine-protein kinase Chk1
  • EC 2.7.11
  • EC 2.7.11.1

Background

Chk1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by Chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHEK1