![Immunohistochemistry: Chk1 Antibody (2F2) [NBP3-26658] - Image of Chk1 Antibody (2F2) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-26220241510613.jpg "Immunohistochemistry: Chk1 Antibody (2F2) [NBP3-26658] - Image of Chk1 Antibody (2F2) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 2F2 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant monoclonal antibody expressed in HEK293F cells |
| Immunogen | A synthesized peptide derived from Human Chk1 [UniProt O14757] |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CHEK1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.4, 150mM NaCl and 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Chk1 Antibody (NBP3-26658)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CHEK1 |
![Western Blot: Chk1 Antibody (2F2) [NBP3-26658] - Positive Western Blot detected in: K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate. All lanes: Chk1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55, 44, 51 kDa Observed band size: 55 kDa](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-282202411442640.jpg "Western Blot: Chk1 Antibody (2F2) [NBP3-26658] - Positive Western Blot detected in: K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate. All lanes: Chk1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55, 44, 51 kDa Observed band size: 55 kDa")
![Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2F2) [NBP3-26658] - Staining of Hela Cells with Chk1 Antibody (2F2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-28220241323640.jpg "Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2F2) [NBP3-26658] - Staining of Hela Cells with Chk1 Antibody (2F2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")