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ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free

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Immunohistochemistry-Paraffin: ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free [NBP2-80667] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of mouse liver using ChemR23/CMKLR1 antibody (clone ...read more
Flow Cytometry: ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free [NBP2-80667] - Staining of resident mouse peritoneal macrophages. Image from the standard format of this antibody.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC
Clone
BZ194
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

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ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free Summary

Immunogen
The immunogen for this antibody was CMKLR1.
Localization
Multi-pass membrane protein with cytoplasmic and extra-cellular domains
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
CMKLR1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Paraffin
Application Notes
For FLOW validation, we tested this antibody on resident mouse peritoneal macrophages and found that it can be used at less than or equal to 0.5 ug per test (cell number should be determined empirically but can range from 10^5 to 10^8cells/test). In IHC-P, we tested it on mouse liver using routine protocol with heat induced antigen retrieval employing 10mM sodium citrate buffer pH 6

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free

  • CHEMERINR
  • chemokine receptor-like 1
  • chemokine-like receptor 1
  • ChemR23
  • cmklr1
  • Dez
  • Gpcr27
  • G-protein coupled receptor ChemR23
  • G-protein coupled receptor DEZ
  • MGC126105
  • MGC126106
  • orphan G-protein coupled receptor, Dez

Background

The 194.7.4 (BZ194)monoclonal antibody reacts to mouse Chemoattractant-like receptor 1 (CMKLR1), also known as ChemR23 and DEZ. CMKLR1 is the receptor for chemerin, a potent chemoattractant. The BZ194 antibody was generated against the amino terminal residues of mouse CMKLR1. In the human CMKLR is found on pDC while in the mouse expression is confined to resident peritoneal macrophages with highest expression on Cd11blo macrophages. Expression is absent on in vitro cultured bone marrow DCs (except on early DCs as defined by CD11c+MHCIIint), and on freshly isolated CD11c+ cells from lymph node, spleen and bone marrow. Mouse CMKLR is thought to play a role in macrophage function as a result of injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CMKLR1