![Western Blot: CENPM Antibody (4C12-2C8) [H00079019-M01] - C22orf18 monoclonal antibody (M01), clone 4C12-2C8 Analysis of C22orf18 expression in K-562.](http://images.novusbio.com/fullsize/CENPM-Antibody-4C12-2C8-Western-Blot-H00079019-M01-img0008.jpg "Western Blot: CENPM Antibody (4C12-2C8) [H00079019-M01] - C22orf18 monoclonal antibody (M01), clone 4C12-2C8 Analysis of C22orf18 expression in K-562.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP |
| Clone | 4C12-2C8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | C22orf18 (AAH00705, 1 a.a. ~ 180 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSVLRPLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNRPRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKLAHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL |
| Localization | Cytoplasmic and Nuclear. Note: Nuclear in non confluent cells and cytoplasmic in confluent or dividing cells. Localizes in the kinetochore domain of centromeres. |
| Specificity | C22orf18 - chromosome 22 open reading frame 18 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CENPM |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: CENPM Antibody (4C12-2C8) [H00079019-M01] - Analysis of monoclonal antibody to MGC861 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CENPM-Antibody-4C12-2C8-Immunocytochemistry-Immunofluorescence-H00079019-M01-img0005.jpg "Immunocytochemistry/Immunofluorescence: CENPM Antibody (4C12-2C8) [H00079019-M01] - Analysis of monoclonal antibody to MGC861 on HeLa cell. Antibody concentration 10 ug/ml.")
![Western Blot: CENPM Antibody (4C12-2C8) [H00079019-M01] - Analysis of CENPM expression in transfected 293T cell line by C22orf18 monoclonal antibody (M01), clone 4C12-2C8.Lane 1: CENPM transfected lysate (Predicted MW: 19.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CENPM-Antibody-4C12-2C8-Western-Blot-H00079019-M01-img0009.jpg "Western Blot: CENPM Antibody (4C12-2C8) [H00079019-M01] - Analysis of CENPM expression in transfected 293T cell line by C22orf18 monoclonal antibody (M01), clone 4C12-2C8.Lane 1: CENPM transfected lysate (Predicted MW: 19.7 KDa).Lane 2: Non-transfected lysate.")
![Immunoprecipitation: CENPM Antibody (4C12-2C8) [H00079019-M01] - Analysis of CENPM transfected lysate using anti-CENPM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CENPM MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/CENPM-Antibody-4C12-2C8-Immunoprecipitation-H00079019-M01-img0006.jpg "Immunoprecipitation: CENPM Antibody (4C12-2C8) [H00079019-M01] - Analysis of CENPM transfected lysate using anti-CENPM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CENPM MaxPab rabbit polyclonal antibody.")
![ELISA: CENPM Antibody (4C12-2C8) [H00079019-M01] - Detection limit for recombinant GST tagged C22orf18 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CENPM-Antibody-4C12-2C8-ELISA-H00079019-M01-img0010.jpg "ELISA: CENPM Antibody (4C12-2C8) [H00079019-M01] - Detection limit for recombinant GST tagged C22orf18 is approximately 0.03ng/ml as a capture antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")