Novus Biologicals products are now on bio-techne.com

Recombinant Human CENPH His Protein

Images

 
SDS-Page: Recombinant Human CENPH Protein [NBP1-72449] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Recombinant Human CENPH His Protein Summary

Description
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 136-247 of Human CENPH

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MLNKLIMKSQ QESWDLEEKL LDIRKKRLQL KQASESKLLE IQTEKNKQKI DLDSMENSER IKIIRQNLQM EIKITTVIQH VFQNLILGSK VNWAEDPALK EIVLQLEKNV DMM

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CENPH
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Application Notes
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
Theoretical MW
15.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human CENPH His Protein

  • CENP-H
  • centromere protein H
  • ICEN35
  • Interphase centromere complex protein 35
  • kinetochore protein CENP-H
  • NNF1
  • PMF1

Background

CENPH, also known as centromere protein H, belongs to the centromere protein H family. This protein is component of the CENPA-NAC (nucleosome-associated) complex that plays a central role in assembly of kinetochore proteins. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Recombinant human CENPH protein, fused to His-tag at N-terminus, was expressed in E.coli and denatured using detergent during a conventional chromatography purification process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP3-13389
Species: Hu
Applications: IHC, IHC-P, WB
NBP3-24732
Species: Hu
Applications: ICC/IF, WB
NB100-338
Species: Ha, Hu, Mar, Mu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IP, PLA, WB
NB100-2359
Species: Hu
Applications: IHC, IP, WB
H00064105-M01
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
H00001059-B01P
Species: Hu
Applications: ICC/IF, WB
NBP1-79664
Species: Hu
Applications: ICC/IF, WB
NBP2-34093
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-82875
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB500-171
Species: Hu, I, Mu, Rt, Xp, Ye
Applications: ChIP, IB, ICC/IF, IHC, IHC-P, Single-Cell Western, WB
NB100-2586
Species: Hu
Applications: ICC/IF, IP, WB
H00378708-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NB100-353
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC/IF, IP, WB
NBP2-37471
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-20053
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB
NB100-563
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB

Publications for CENPH Protein (NBP1-72449) (0)

There are no publications for CENPH Protein (NBP1-72449).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CENPH Protein (NBP1-72449) (0)

There are no reviews for CENPH Protein (NBP1-72449). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for CENPH Protein (NBP1-72449) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CENPH Products

Research Areas for CENPH Protein (NBP1-72449)

Find related products by research area.

Blogs on CENPH

There are no specific blogs for CENPH, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human CENPH His Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol CENPH