![Western Blot: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Analysis of MBD5 expression in transfected 293T cell line by MBD5 monoclonal antibody (M01), clone 4A12-1B6.Lane 1: MBD5 transfected lysate(24.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MBD5-Antibody-4A12-1B6-Western-Blot-H00055777-M01-img0005.jpg "Western Blot: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Analysis of MBD5 expression in transfected 293T cell line by MBD5 monoclonal antibody (M01), clone 4A12-1B6.Lane 1: MBD5 transfected lysate(24.3 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 4A12-1B6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | MBD5 (AAH14534.1, 1 a.a. ~ 229 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MFPPTANMLLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGMVLLEKSTQRY |
| Specificity | MBD5 - methyl-CpG binding domain protein 5 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MBD5 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
| Publication using H00055777-M01 | Applications | Species |
|---|---|---|
| Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01 |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Analysis of monoclonal antibody to MBD5 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MBD5-Antibody-4A12-1B6-Immunocytochemistry-Immunofluorescence-H00055777-M01-img0003.jpg "Immunocytochemistry/Immunofluorescence: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Analysis of monoclonal antibody to MBD5 on HeLa cell . Antibody concentration 10 ug/ml.")
![Sandwich ELISA: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Detection limit for recombinant GST tagged MBD5 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MBD5-Antibody-4A12-1B6-Sandwich-ELISA-H00055777-M01-img0004.jpg "Sandwich ELISA: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Detection limit for recombinant GST tagged MBD5 is 0.03 ng/ml as a capture antibody.")
![Western Blot Growth Hormone Antibody [Unconjugated]](https://images.novusbio.com/images2/Growth_Hormone_AF1067_Western_Blot_20961.jpg)
![ELISA Growth Hormone Antibody [Unconjugated]](https://images.novusbio.com/images2/Growth_Hormone_AF1067_ELISA_22201.jpg)
![Neutralization Growth Hormone Antibody [Unconjugated]](https://images.novusbio.com/images2/Growth_Hormone_AF1067_Block_Neutralize_8751.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")