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CD99 Antibody (OTI1D5)

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Western Blot: CD99 Antibody (1D5) [NBP2-46181] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD99.
Western Blot: CD99 Antibody (1D5) [NBP2-46181] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD99.
Immunohistochemistry-Paraffin: CD99 Antibody (OTI1D5) [NBP2-46181] - CD99 Antibody (1D5) [NBP2-46181] - Analysis of Human pancreas tissue.
Flow Cytometry: CD99 Antibody (1D5) [NBP2-46181] - Analysis of Jurkat cells, using CD99 antibody (Red), compared to a nonspecific negative control antibody (Blue). Dilution: 1:50
Western Blot: CD99 Antibody (1D5) [NBP2-46181] - Analysis of A549 cell lysate (35ug).
Immunohistochemistry-Paraffin: CD99 Antibody (OTI1D5) [NBP2-46181] - CD99 Antibody (1D5) [NBP2-46181] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry-Paraffin: CD99 Antibody (OTI1D5) [NBP2-46181] - CD99 Antibody (1D5) [NBP2-46181] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry-Paraffin: CD99 Antibody (OTI1D5) [NBP2-46181] - CD99 Antibody (1D5) [NBP2-46181] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI1D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71197AF350
Alexa Fluor 405
NBP2-71197AF405
Alexa Fluor 488
NBP2-71197AF488
Alexa Fluor 532
NBP2-71197AF532
Alexa Fluor 594
NBP2-71197AF594
Alexa Fluor 647
NBP2-71197AF647
Alexa Fluor 700
NBP2-71197AF700
Alexa Fluor 750
NBP2-71197AF750
Allophycocyanin
NBP2-71197APC
Biotin
NBP2-71197B
CoraFluor 1
NBP2-71197CL1
DyLight 350
NBP2-71197UV
DyLight 405
NBP2-71197V
DyLight 488
NBP2-71197G
DyLight 550
NBP2-71197R
DyLight 594
NBP2-71197DL594
DyLight 650
NBP2-71197C
DyLight 680
NBP2-71197FR
DyLight 755
NBP2-71197IR
FITC
NBP2-71197F
HRP
NBP2-71197H
Janelia Fluor 525
NBP2-71197JF525
Janelia Fluor 549
NBP2-71197JF549
Janelia Fluor 585
NBP2-71197JF585
Janelia Fluor 635
NBP2-71197JF635
Janelia Fluor 646
NBP2-71197JF646
Janelia Fluor 669
NBP2-71197JF669
PE
NBP2-71197PE
PerCP
NBP2-71197PCP
mFluor Violet 450 SE
NBP2-71197MFV450
mFluor Violet 500 SE
NBP2-71197MFV500
mFluor Violet 610 SE
NBP2-71197MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71197
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CD99 Antibody (OTI1D5) Summary

Immunogen
Full length human recombinant protein of human CD99 (NP_002405) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD99
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
16.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD99 Antibody (OTI1D5)

  • 12E7
  • CD99 antigenY homolog
  • CD99 molecule
  • CD99
  • E2 antigen
  • HBA71
  • MIC2 (monoclonal 12E7)
  • MIC2
  • MIC2Y
  • MSK5X
  • pilr-1
  • PILR-L
  • Protein MIC2
  • surface antigen MIC2
  • T-cell surface glycoprotein E2

Background

CD99 is encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD99