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CD99 Antibody (MIC2/877)

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Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/877) [NBP2-44695] - Human Testicular Carcinoma & stained with CD99 Monoclonal Antibody (MIC2/877).
Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/877) [NBP2-44695] - Human Testicular Carcinoma & stained with CD99 Monoclonal Antibody (MIC2/877).
Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/877) [NBP2-44695] - Human Ewing's Sarcoma stained with CD99 Monoclonal Antibody (MIC2/877).
Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/877) [NBP2-44695] - Human Ovarian Carcinoma & stained with CD99 Monoclonal Antibody (MIC2/877).

Product Details

Summary
Reactivity Hu, Rt(-)Species Glossary
Applications Flow, ICC/IF, IHC, ICC/IF
Clone
MIC2/877
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47895AF350
Alexa Fluor 405
NBP2-47895AF405
Alexa Fluor 488
NBP2-47895AF488
Alexa Fluor 532
NBP2-47895AF532
Alexa Fluor 594
NBP2-47895AF594
Alexa Fluor 647
NBP2-47895AF647
Alexa Fluor 700
NBP2-47895AF700
Alexa Fluor 750
NBP2-47895AF750
Allophycocyanin
NBP2-47895APC
Allophycocyanin/Cy7
NBP2-47895APCCY7
Biotin
NBP2-47895B
CoraFluor 1
NBP2-47895CL1
DyLight 350
NBP2-47895UV
DyLight 405
NBP2-47895V
DyLight 488
NBP2-47895G
DyLight 550
NBP2-47895R
DyLight 594
NBP2-47895DL594
DyLight 650
NBP2-47895C
DyLight 680
NBP2-47895FR
DyLight 755
NBP2-47895IR
FITC
NBP2-47895F
HRP
NBP2-47895H
Janelia Fluor 525
NBP2-47895JF525
Janelia Fluor 549
NBP2-47895JF549
Janelia Fluor 585
NBP2-47895JF585
Janelia Fluor 635
NBP2-47895JF635
Janelia Fluor 646
NBP2-47895JF646
Janelia Fluor 669
NBP2-47895JF669
PE
NBP2-47895PE
PE/Atto594
NBP2-47895PEATT594
PE/Cy5.5
NBP2-47895PECY55
PE/Cy7
NBP2-47895PECY7
PerCP
NBP2-47895PCP
mFluor Violet 450 SE
NBP2-47895MFV450
mFluor Violet 500 SE
NBP2-47895MFV500
mFluor Violet 610 SE
NBP2-47895MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-47895-0.1mg
IHC-Prediluted
NBP2-48034
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CD99 Antibody (MIC2/877) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47895)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant full-length human CD99 protein (Uniprot: P14209)
Localization
Cell surface
Marker
Ewing's Sarcoma Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD99
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reactivity Notes

Does not react with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for CD99 Antibody (MIC2/877)

  • 12E7
  • CD99 antigenY homolog
  • CD99 molecule
  • CD99
  • E2 antigen
  • HBA71
  • MIC2 (monoclonal 12E7)
  • MIC2
  • MIC2Y
  • MSK5X
  • pilr-1
  • PILR-L
  • Protein MIC2
  • surface antigen MIC2
  • T-cell surface glycoprotein E2

Background

CD99 is encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CD99
Uniprot