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CD84/SLAMF5 Antibody (152-1D5) [Janelia Fluor® 525]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, ICC/IF
Clone
152-1D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 525

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-11538AF350
Alexa Fluor 405
NBP3-11538AF405
Alexa Fluor 488
NBP3-11538AF488
Alexa Fluor 532
NBP3-11538AF532
Alexa Fluor 594
NBP3-11538AF594
Alexa Fluor 647
NBP3-11538AF647
Alexa Fluor 700
NBP3-11538AF700
Alexa Fluor 750
NBP3-11538AF750
Allophycocyanin
NBP3-11538APC
Biotin
NBP3-11538B
CoraFluor 1
NBP3-11538CL1
DyLight 350
NBP3-11538UV
DyLight 405
NBP3-11538V
DyLight 488
NBP3-11538G
DyLight 550
NBP3-11538R
DyLight 594
NBP3-11538DL594
DyLight 650
NBP3-11538C
DyLight 680
NBP3-11538FR
DyLight 755
NBP3-11538IR
FITC
NBP3-11538F
HRP
NBP3-11538H
Janelia Fluor 549
NBP3-11538JF549
Janelia Fluor 585
NBP3-11538JF585
Janelia Fluor 635
NBP3-11538JF635
Janelia Fluor 646
NBP3-11538JF646
Janelia Fluor 669
NBP3-11538JF669
PE
NBP3-11538PE
PerCP
NBP3-11538PCP
Unconjugated
NBP2-44345-0.1mg
mFluor Violet 450 SE
NBP3-11538MFV450
mFluor Violet 500 SE
NBP3-11538MFV500
mFluor Violet 610 SE
NBP3-11538MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44345-0.1mg
Azide and BSA Free
NBP3-11538
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CD84/SLAMF5 Antibody (152-1D5) [Janelia Fluor® 525] Summary

Additional Information
Clone 152-1D5 was used by HLDA to establish CD designation.
Immunogen
Spleen cells of a patient with hairy cell leukemia
Localization
Cell Surface
Specificity
Recognizes a protein of 74kDa, identified as CD84 (Workshop V; Code A085, C057). It is expressed on mature B cells and on B-cell lines, including pre-B-cell lines, but not on plasma cell lines. Immunohistochemical studies demonstrated that CD84 strongly expressed on tissues macrophages. CD84 is also highly expressed on platelets and, at low levels on peripheral blood T cells. It is a highly N-glycosylated protein and belongs to immunoglobulin superfamily. It may play a role in leukocyte activation.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD84
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CD84/SLAMF5 Antibody (152-1D5) [Janelia Fluor® 525]

  • CD84 antigen (leukocyte antigen)
  • CD84 antigen
  • CD84 molecule
  • CD84
  • Cell surface antigen MAX.3
  • DKFZp781E2378
  • hCD84
  • hly9-beta
  • leucocyte differentiation antigen CD84
  • leukocyte antigen CD84
  • Leukocyte differentiation antigen CD84
  • Ly-9B
  • mCD84
  • Signaling lymphocytic activation molecule 5
  • SLAM family member 5
  • SLAMF5
  • SLAMF5LY9B

Background

CD84 is a 64-82 kD glycoprotein. It is a member of the SLAM (CD150) family, a CD2 subset of the Ig superfamily, also known as SLAMF5 or Ly9b. CD84 is expressed on B cells, monocytes, thymocytes, subset of T cells, and platelets. CD84 functions as a homophilic adhesion molecule and enhances T cell activation and cytokine production. CD84 is expressed on mature B cells and B cell lines but not on plasma cell lines. Immunohistochemical studies demonstrate that it strongly stains tissue macrophages. It is also expressed on platelets and at low levels on blood T cells. Cellular expression does not significantly increase after activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CD84