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CD59 Antibody (YTH53.1)

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Immunohistochemistry-Paraffin: CD59 Antibody (YTH53.1) [NB100-65537] Immunoperoxidase staining of formalin fixed, paraffin embedded human placenta with Rat anti Human CD59
Immunohistochemistry-Paraffin: CD59 Antibody (YTH53.1) [NB100-65537] Immunoperoxidase staining of formalin fixed, paraffin embedded human placenta with Rat anti Human CD59
Flow Cytometry: CD59 Antibody (YTH53.1) [NB100-65537] - Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD59 (NB100-65537).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Func, IHC
Clone
YTH53.1
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-65537AF350
Alexa Fluor 405
NB100-65537AF405
Alexa Fluor 488
NB100-65537AF488
Alexa Fluor 532
NB100-65537AF532
Alexa Fluor 594
NB100-65537AF594
Alexa Fluor 647
NB100-65537AF647
Alexa Fluor 700
NB100-65537AF700
Alexa Fluor 750
NB100-65537AF750
Allophycocyanin
NB100-65537APC
Allophycocyanin/Cy7
NB100-65537APCCY7
Biotin
NB100-65537B
CoraFluor 1
NB100-65537CL1
DyLight 350
NB100-65537UV
DyLight 405
NB100-65537V
DyLight 488
NB100-65537G
DyLight 550
NB100-65537R
DyLight 594
NB100-65537DL594
DyLight 650
NB100-65537C
DyLight 680
NB100-65537FR
DyLight 755
NB100-65537IR
FITC
NB100-65537F
HRP
NB100-65537H
Janelia Fluor 525
NB100-65537JF525
Janelia Fluor 549
NB100-65537JF549
Janelia Fluor 585
NB100-65537JF585
Janelia Fluor 635
NB100-65537JF635
Janelia Fluor 646
NB100-65537JF646
Janelia Fluor 669
NB100-65537JF669
PE
NB100-65537PE
PE/Atto594
NB100-65537PEATT594
PE/Cy5.5
NB100-65537PECY55
PE/Cy7
NB100-65537PECY7
PerCP
NB100-65537PCP
mFluor Violet 450 SE
NB100-65537MFV450
mFluor Violet 500 SE
NB100-65537MFV500
mFluor Violet 610 SE
NB100-65537MFV610
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CD59 Antibody (YTH53.1) Summary

Additional Information
Clone YTH53.1 was used by HLDA to establish CD designation.
Immunogen
Human Peripheral Blood T cells
Localization
Cell membrane; lipid anchor; GPI anchor.
Specificity
NB100-65537 recognizes the human CD59 cell surface antigen, an 18-20kD GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. Clone YTH53.1 has been shown to block the normal function of CD59. Removal of sodium azide is recommended prior to use in functional assays. CD59 is expressed by erythrocytes, and therefore staining in whole blood systems requires a higher concentration of antibody.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
CD59
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50 - 1:100
  • Functional
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:5 - 1:10
  • Western Blot 1:100 - 1:2000
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
Control
CD59 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD59 Antibody (YTH53.1)

  • 16.3A5
  • 1F5 antigen
  • 1F5
  • 20 kDa homologous restriction factor
  • CD59 antigen
  • CD59 antigen, complement regulatory protein
  • CD59 glycoprotein
  • CD59 molecule, complement regulatory protein
  • CD59
  • EJ16
  • EJ30
  • EJ30, EL32 and G344)
  • EL32
  • FLJ38134
  • FLJ92039
  • G344
  • HRF20
  • HRF-20
  • human leukocyte antigen MIC11
  • Ly-6-like protein
  • lymphocytic antigen CD59/MEM43
  • MACIF
  • MAC-inhibitory protein
  • MAC-IP
  • MEM43 antigen
  • MEM43
  • membrane attack complex (MAC) inhibition factor
  • Membrane attack complex inhibition factor
  • Membrane inhibitor of reactive lysis
  • MGC2354
  • MIC11
  • MIC11MSK21
  • MIN1
  • MIN2
  • MIN3
  • MIRL
  • p18-20
  • Protectin
  • surface anitgen recognized by monoclonal 16.3A5
  • T cell-activating protein

Background

CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD59 Antibody
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NB100-65537
CD59 Antibody (YTH53.1)
Species: Hu
Applications: WB, Flow, Func, IHC

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WB Video Protocol

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Control Lysate(s)

CD59 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD59
Uniprot