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Human CD55/DAF ELISA Kit (Colorimetric)

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ELISA: Human CD55/DAF ELISA Kit (Colorimetric) [NBP2-80311] - Example standard curve for Human CD55/DAF ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP
Suitable Sample Type
Serum
Standard Curve Range
125-8000 pg/ml (example only; lot dependent)
Sensitivity
91.65 pg/ml

Order Details

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Human CD55/DAF ELISA Kit (Colorimetric) Summary

Specificity
Recognizes both recombinant and natural Human CD55/DAF.
Standard Curve Range
125-8000 pg/ml (example only; lot dependent)
Sensitivity
91.65 pg/ml
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 3.6 %
Intra-Assay
CV% < 3
Spike Recovery
82 -86%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
CD55

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. 1. 96 well microplate coated with Capture Antibody
  2. 2. Detection Antibody conjugated to HRP
  3. 3. Standards
  4. 4. Wash Buffer Concentrate
  5. 5. Dilution Buffer Concentrate
  6. 6. Color Reagent A
  7. 7. Color Reagent B
  8. 8. Stop Solution

Alternate Names for Human CD55/DAF ELISA Kit (Colorimetric)

  • CD55 antigen
  • CD55 molecule, decay accelerating factor for complement (Cromer blood group)
  • CD55
  • CR
  • CRdecay accelerating factor for complement (CD55, Cromer blood group system)
  • CROMDAFcomplement decay-accelerating factor
  • DAF
  • decay accelerating factor for complement
  • TC

Background

CD55 encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CD55