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CD55/DAF Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-41295AF350
Alexa Fluor 405
NBP2-41295AF405
Alexa Fluor 488
NBP2-41295AF488
Alexa Fluor 532
NBP2-41295AF532
Alexa Fluor 594
NBP2-41295AF594
Alexa Fluor 647
NBP2-41295AF647
Alexa Fluor 700
NBP2-41295AF700
Alexa Fluor 750
NBP2-41295AF750
Biotin
NBP2-41295B
DyLight 350
NBP2-41295UV
DyLight 405
NBP2-41295V
DyLight 488
NBP2-41295G
DyLight 550
NBP2-41295R
DyLight 594
NBP2-41295DL594
DyLight 650
NBP2-41295C
DyLight 680
NBP2-41295FR
DyLight 755
NBP2-41295IR
FITC
NBP2-41295F
HRP
NBP2-41295H
Janelia Fluor 525
NBP2-41295JF525
Janelia Fluor 549
NBP2-41295JF549
Janelia Fluor 646
NBP2-41295JF646
Janelia Fluor 669
NBP2-41295JF669
PerCP
NBP2-41295PCP
Unconjugated
NBP2-41295
mFluor Violet 450 SE
NBP2-41295MFV450
mFluor Violet 500 SE
NBP2-41295MFV500
mFluor Violet 610 SE
NBP2-41295MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-41295
BSA Free
NBP2-41295
BSA Free
NBP2-41295-0.025mg
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CD55/DAF Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DAF. The immunogen is located within amino acids 360 - 410 of DAF. Amino Acid Squence: DRSSLQSRTPGFKRSFH
Specificity
DAF antibody is human specific. At least two isoforms of DAF are known to exist; this antibody will only detect the longest isoform.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD55
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Peptide affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for CD55/DAF Antibody [CoraFluor™ 1]

  • CD55 antigen
  • CD55 molecule, decay accelerating factor for complement (Cromer blood group)
  • CD55
  • CR
  • CRdecay accelerating factor for complement (CD55, Cromer blood group system)
  • CROMDAFcomplement decay-accelerating factor
  • DAF
  • decay accelerating factor for complement
  • TC

Background

CD55 encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD55/DAF Antibody
[CoraFluor™ 1]
NBP2-41295CL1
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Species: Hu
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Bioinformatics

Gene Symbol CD55