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CD55/DAF Antibody (583905) [Unconjugated]

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Western blot shows lysates of mouse splenocytes. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse CD55/DAF Monoclonal Antibody (Catalog # MAB5376) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD55/DAF at approximately 60 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse splenocytes. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse CD55/DAF Monoclonal Antibody (Catalog # MAB5376) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody ...read more
Western blot shows lysates of mouse spleen tissue and mouse splenocytes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CD55/DAF Monoclonal Antibody (Catalog # MAB5376) followed by HRP-conjugated Anti-Rat IgG ...read more
Mouse splenocytes were stained with Rat Anti-Mouse CD55/DAF Monoclonal Antibody (Catalog # MAB5376, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
583905
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB5376U-100UG
Alexa Fluor 405
FAB5376V-100UG
Alexa Fluor 488
FAB5376G-100UG
Alexa Fluor 532
FAB5376X
Alexa Fluor 594
FAB5376T-100UG
Alexa Fluor 647
FAB5376R-100UG
Alexa Fluor 700
FAB5376N-100UG
Alexa Fluor 750
FAB5376S-100UG
Allophycocyanin
FAB5376A
Allophycocyanin/Cy7
FAB5376APCCY7
Biotin
FAB5376B
CoraFluor 1
FAB5376CL1
DyLight 350
FAB5376D
DyLight 405
FAB5376E
DyLight 488
FAB5376K
DyLight 550
FAB5376L
DyLight 594
FAB5376M
DyLight 650
FAB5376W
DyLight 680
FAB5376Y
DyLight 755
FAB5376Z
FITC
FAB5376F
HRP
FAB5376H
Janelia Fluor 525
FAB5376JF525
Janelia Fluor 549
FAB5376I
Janelia Fluor 635
FAB5376JF635
Janelia Fluor 646
FAB5376J
Janelia Fluor 669
FAB5376JF669
PE/Atto594
FAB5376PEATT594
PE/Cy5.5
FAB5376PECY55
PE/Cy7
FAB5376PECY7
PerCP
FAB5376C
mFluor Violet 450 SE
FAB5376MFV450
mFluor Violet 500 SE
FAB5376MFV500
mFluor Violet 610 SE
FAB5376MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5376-SP
Carrier Free
FAB5376V-100UG
Carrier Free
FAB5376U-100UG
Carrier Free
FAB5376T-100UG
Carrier Free
FAB5376S-100UG
Carrier Free
FAB5376R-100UG
Carrier Free
FAB5376N-100UG
Carrier Free
FAB5376G-100UG
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CD55/DAF Antibody (583905) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD55/DAF
Met1-Pro359
Accession # Q61475
Specificity
Detects mouse CD55/DAF in direct ELISAs and Western blots.  In direct ELISAs, no cross-reactivity with recombinant human CD55 or recombinant mouse CD97 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CD55
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 2 ug/mL
Publications
Read Publication using
MAB5376 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD55/DAF Antibody (583905) [Unconjugated]

  • CD55 antigen
  • CD55 molecule, decay accelerating factor for complement (Cromer blood group)
  • CD55
  • CR
  • CRdecay accelerating factor for complement (CD55, Cromer blood group system)
  • CROMDAFcomplement decay-accelerating factor
  • DAF
  • decay accelerating factor for complement
  • TC

Background

CD55 (Decay-accelarating factor/DAF) is a glycoprotein member of the RCA family of molecules. It is found on blood cells, epithelium and endothelium, and serves both as a receptor for CD97, and a negative regulator of the C3 convertases, C4b2a and C3bBb. Mature mouse CD55 is the product of two genes that arose by duplication. There is a 55-60 kDa, 356 amino acid (aa), GPI-linked form that is ubiquitously expressed. This molecule contains four SUSHI domains (aa 35-285), a Ser/Thr-rich region (aa 288-362), and a GPI-anchor at Gly362. There is also a 50 kDa, 379 aa, type I transmembrane form that is testis-associated. It shows the same domain architecture and is 93% aa identical to the GPI-form. At least four GPI gene isoforms exist. They diverge after Ile285 and show deletions and substitutions. Over aa 35-359, mouse CD55 is 66% and 50% aa identical to rat and human CD55, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD55/DAF Antibody
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MAB5376
CD55/DAF Antibody (583905) [Unconjugated]
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Publications for CD55/DAF Antibody (MAB5376)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol CD55
Uniprot