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Human CD35 ELISA Kit (Colorimetric)

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ELISA: Human CD21/CD35 ELISA Kit (Colorimetric) [NBP3-11782] - Samples were spiked with high concentrations of Human CD21/CD35 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human CD21/CD35 ELISA Kit (Colorimetric) [NBP3-11782] - Samples were spiked with high concentrations of Human CD21/CD35 and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Human CD21/CD35 ELISA Kit (Colorimetric) [NBP3-11782] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL

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Human CD35 ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 5.66
Intra-Assay
%CV < 6.12
Spike Recovery
87 - 108%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CR1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Antibody Diluent
  2. Concentrated Biotinylated Detection Antibody
  3. Concentrated HRP Conjugate
  4. Concentrated Wash Buffer
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate
  7. Plate Sealer
  8. Reference Standard & Sample Diluent
  9. Reference Standard
  10. Stop Solution
  11. Substrate Reagent

Alternate Names for Human CD35 ELISA Kit (Colorimetric)

  • C3BR
  • CD35
  • complement component (3b/4b) receptor 1 (Knops blood group)
  • CR1
  • KN

Background

CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Human CD35 ELISA Kit (Colorimetric) Manual
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Bioinformatics

Gene Symbol CR1