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CD35 Antibody (To5) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC
Clone
To5
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08279AF350
Alexa Fluor 405
NBP3-08279AF405
Alexa Fluor 488
NBP3-08279AF488
Alexa Fluor 532
NBP3-08279AF532
Alexa Fluor 594
NBP3-08279AF594
Alexa Fluor 647
NBP3-08279AF647
Alexa Fluor 700
NBP3-08279AF700
Alexa Fluor 750
NBP3-08279AF750
Allophycocyanin
NBP3-08279APC
Biotin
NBP3-08279B
CoraFluor 1
NBP3-08279CL1
DyLight 350
NBP3-08279UV
DyLight 405
NBP3-08279V
DyLight 488
NBP3-08279G
DyLight 550
NBP3-08279R
DyLight 594
NBP3-08279DL594
DyLight 650
NBP3-08279C
DyLight 680
NBP3-08279FR
DyLight 755
NBP3-08279IR
FITC
NBP3-08279F
HRP
NBP3-08279H
Janelia Fluor 525
NBP3-08279JF525
Janelia Fluor 549
NBP3-08279JF549
Janelia Fluor 585
NBP3-08279JF585
Janelia Fluor 635
NBP3-08279JF635
Janelia Fluor 646
NBP3-08279JF646
PE
NBP3-08279PE
PerCP
NBP3-08279PCP
Unconjugated
NBP3-07752-100ug
mFluor Violet 450 SE
NBP3-08279MFV450
mFluor Violet 500 SE
NBP3-08279MFV500
mFluor Violet 610 SE
NBP3-08279MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-07752-100ug
Azide and BSA Free
NBP3-08279
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CD35 Antibody (To5) [Janelia Fluor® 669] Summary

Immunogen
Recombinant human CD35 protein (Uniprot: P17927)
Localization
Cell Surface
Marker
Follicular Dendritic Cell Marker
Specificity
Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This monoclonal antibody does not block CR1 activity. It is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This monoclonal antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CR1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CD35 Antibody (To5) [Janelia Fluor® 669]

  • C3BR
  • CD35
  • complement component (3b/4b) receptor 1 (Knops blood group)
  • CR1
  • KN

Background

CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CR1