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CD35 Antibody (E11) [DyLight 550]

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Product Details

Summary
Reactivity Hu, Pm, Pm, Pm-Cm, RMSpecies Glossary
Applications Flow, ICC/IF, IHC
Clone
E11
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 550

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-33080AF350
Alexa Fluor 405
NBP2-33080AF405
Alexa Fluor 488
NBP2-33080AF488
Alexa Fluor 532
NBP2-33080AF532
Alexa Fluor 594
NBP2-33080AF594
Alexa Fluor 647
NBP2-33080AF647
Alexa Fluor 700
NBP2-33080AF700
Alexa Fluor 750
NBP2-33080AF750
Allophycocyanin
NBP2-33080APC
Biotin
NBP2-33080B
DyLight 350
NBP2-33080UV
DyLight 405
NBP2-33080V
DyLight 488
NBP2-33080G
DyLight 594
NBP2-33080DL594
DyLight 650
NBP2-33080C
DyLight 680
NBP2-33080FR
DyLight 755
NBP2-33080IR
FITC
NBP2-33080F
HRP
NBP2-33080H
Janelia Fluor 549
NBP2-33080JF549
Janelia Fluor 646
NBP2-33080JF646
PE
NBP2-33080PE
PerCP
NBP2-33080PCP
Unconjugated
NBP2-29460
mFluor Violet 450 SE
NBP2-33080MFV450
mFluor Violet 500 SE
NBP2-33080MFV500
mFluor Violet 610 SE
NBP2-33080MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-29460
Azide and BSA Free
NBP2-33080
IHC-Prediluted
NBP2-44617
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CD35 Antibody (E11) [DyLight 550] Summary

Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Additional Information
Clone E11 was used by HLDA to establish CD designation.
Immunogen
Intact human monocytes
Localization
Cell surface
Marker
Follicular Dendritic Cell Marker
Specificity
Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This monoclonal antibody is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This monoclonal antibody is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This monoclonal antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CR1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CD35 Antibody (E11) [DyLight 550]

  • C3BR
  • CD35
  • complement component (3b/4b) receptor 1 (Knops blood group)
  • CR1
  • KN

Background

CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CR1