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CD35 Antibody (8C12) [Janelia Fluor® 585]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready
Clone
8C12
Clonality
Monoclonal
Host
Rabbit
Conjugate
Janelia Fluor 585

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-52668AF350
Alexa Fluor 405
NBP2-52668AF405
Alexa Fluor 488
NBP2-52668AF488
Alexa Fluor 532
NBP2-52668AF532
Alexa Fluor 594
NBP2-52668AF594
Alexa Fluor 647
NBP2-52668AF647
Alexa Fluor 700
NBP2-52668AF700
Alexa Fluor 750
NBP2-52668AF750
Allophycocyanin
NBP2-52668APC
Allophycocyanin/Cy7
NBP2-52668APCCY7
Biotin
NBP2-52668B
CoraFluor 1
NBP2-52668CL1
DyLight 350
NBP2-52668UV
DyLight 405
NBP2-52668V
DyLight 488
NBP2-52668G
DyLight 550
NBP2-52668R
DyLight 594
NBP2-52668DL594
DyLight 650
NBP2-52668C
DyLight 680
NBP2-52668FR
DyLight 755
NBP2-52668IR
FITC
NBP2-52668F
HRP
NBP2-52668H
Janelia Fluor 525
NBP2-52668JF525
Janelia Fluor 549
NBP2-52668JF549
Janelia Fluor 635
NBP2-52668JF635
Janelia Fluor 646
NBP2-52668JF646
Janelia Fluor 669
NBP2-52668JF669
PE
NBP2-52668PE
PE/Atto594
NBP2-52668PEATT594
PE/Cy5.5
NBP2-52668PECY55
PE/Cy7
NBP2-52668PECY7
PerCP
NBP2-52668PCP
Unconjugated
NBP2-52668-0.2mg
mFluor Violet 450 SE
NBP2-52668MFV450
mFluor Violet 500 SE
NBP2-52668MFV500
mFluor Violet 610 SE
NBP2-52668MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-52668-0.2mg
Azide and BSA Free
NBP2-52668-0.2mg
Azide and BSA Free
NBP2-52668-0.025mg
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CD35 Antibody (8C12) [Janelia Fluor® 585] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Purified murine CR1 protein.
Specificity
Recognises an epitope on CD35 (CR1, 190 kDa), but not on CD21 (CR2, 145-150 kDa), which are alternative splice variants of primary Cr2 gene transcripts.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
CR1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CD35 Antibody (8C12) [Janelia Fluor® 585]

  • C3BR
  • CD35
  • complement component (3b/4b) receptor 1 (Knops blood group)
  • CR1
  • KN

Background

CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CR1