| Reactivity | HuSpecies Glossary |
| Applications | Flow |
| Clone | 594708 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Allophycocyanin |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human CD35 Gln42-Asp1971 (His1208Arg) Accession # CAA68755 |
| Specificity | Detects human CD35 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat CSMD1, recombinant human (rh) CSMD2, rhCSMD3, rhHABP1/C1QBP, or rhComplement Factor H is observed. |
| Source | N/A |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CR1 |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Protect from light. Do not freeze.
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| Buffer | Supplied in a saline solution containing BSA and Sodium Azide. |
| Preservative | Sodium Azide |
CD35, also known as Complement Receptor 1 (CR1), is a 220-300 kDa N-glycosylated member of the RCA (Regulators of Complement Activation) family of proteins. CD35 binds and internalizes particles and immune complexes that are opsonized with MBL or complement components C3b, C3i, C4b, or C1q. CD35 additionally protects the cell from complement-mediated lysis by serving as a cofactor for Factor I and inhibiting the C3 and C5 convertases. The extracellular domain (ECD) of human CD35 contains 30 tandem SCR/SUSHI repeats. A soluble form of the CD35 ECD circulates in the serum. A mouse ortholog of human CD35 has not been described, although alternate splicing of mouse CD21/CR2 generates a protein with homology to some SCR repeats of human CD35. Cell surface CD35 is widely expressed on hematopoietic cells.
![CD35 Antibody (594708) [Allophycocyanin]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "CD35 Antibody (594708) [Allophycocyanin]")
Secondary Antibodies |
Isotype Controls |
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How To Identify B Cell Subsets Using Flow Cytometry By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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