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CD30 Ligand/TNFSF8 Antibody (RM153) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, B/N, Func
Clone
RM153
Clonality
Monoclonal
Host
Rabbit
Conjugate
Janelia Fluor 549

Order Details

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CD30 Ligand/TNFSF8 Antibody (RM153) [Janelia Fluor® 549] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was raised by immunising Sprague Dawley rats with the CHO cells stably transfected with murine CD30 Ligand/TNFSF8 and fusing the splenocytes with P3U1 myeloma cells.
Specificity
This antibody is specific for murine CD30 Ligand/TNFSF8, a 40-kDa type II transmembrane protein member of the TNF superfamily. Interaction of CD30 with CD30 Ligand/TNFSF8 plays an important role in T cell activation/costimulation.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
TNFSF8
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Block/Neutralize
  • Flow Cytometry
  • Functional Assay
  • Immunocytochemistry/ Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CD30 Ligand/TNFSF8 Antibody (RM153) [Janelia Fluor® 549]

  • CD153 antigen
  • CD153
  • CD30 antigen ligand
  • CD30 Ligand
  • CD30L
  • CD30-L
  • CD30LCD30 ligand
  • CD30LGMGC138144
  • TNFSF8
  • tumor necrosis factor (ligand) superfamily, member 8
  • tumor necrosis factor ligand superfamily member 8

Background

CD153 is a 39-40 kD type II membrane glycoprotein, also known as CD30 ligand or CD30L. This TNF superfamily member is expressed on activated T cells. CD153 has been reported to stimulate B-cell proliferation, antigen-specific antibody production, polyclonal immunoglobulin secretion, and T cell activation and proliferation. The RM153 antibody inhibits binding of CD153 to CD30.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TNFSF8