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CD21 Antibody (CR2/3124R)

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Immunocytochemistry/Immunofluorescence: CD21 Antibody (CR2/3124R) [NBP3-07754] - Immunofluorescence staining of PFA-fixed MOLT4 cells using CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by goat anti- Mouse IgG-CF488 (green). Nuclei are stained with RedDot.
Immunocytochemistry/ Immunofluorescence: CD21 Antibody (CR2/3124R) [NBP3-07754] - Immunofluorescence staining of PFA-fixed MOLT4 cells using CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by goat anti- ...read more
Immunohistochemistry-Paraffin: CD21 Antibody (CR2/3124R) [NBP3-07754] - Formalin-fixed, paraffin-embedded human Tonsil Dendritic stained with CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R).
Flow Cytometry: CD21 Antibody (CR2/3124R) [NBP3-07754] - Flow Cytometric Analysis of PFA-fixed MOLT4 cells. CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
CR2/3124R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08297AF350
Alexa Fluor 405
NBP3-08297AF405
Alexa Fluor 488
NBP3-08297AF488
Alexa Fluor 532
NBP3-08297AF532
Alexa Fluor 594
NBP3-08297AF594
Alexa Fluor 647
NBP3-08297AF647
Alexa Fluor 700
NBP3-08297AF700
Alexa Fluor 750
NBP3-08297AF750
Allophycocyanin
NBP3-08297APC
Biotin
NBP3-08297B
CoraFluor 1
NBP3-08297CL1
DyLight 350
NBP3-08297UV
DyLight 405
NBP3-08297V
DyLight 488
NBP3-08297G
DyLight 550
NBP3-08297R
DyLight 594
NBP3-08297DL594
DyLight 650
NBP3-08297C
DyLight 680
NBP3-08297FR
DyLight 755
NBP3-08297IR
FITC
NBP3-08297F
HRP
NBP3-08297H
Janelia Fluor 525
NBP3-08297JF525
Janelia Fluor 549
NBP3-08297JF549
Janelia Fluor 585
NBP3-08297JF585
Janelia Fluor 635
NBP3-08297JF635
Janelia Fluor 646
NBP3-08297JF646
Janelia Fluor 669
NBP3-08297JF669
PE
NBP3-08297PE
PerCP
NBP3-08297PCP
mFluor Violet 450 SE
NBP3-08297MFV450
mFluor Violet 500 SE
NBP3-08297MFV500
mFluor Violet 610 SE
NBP3-08297MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-08297
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CD21 Antibody (CR2/3124R) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08297)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fragment (around aa 142-240) of human CR2 (CD21) protein (exact sequence is proprietary) (Uniprot: P20023)
Localization
Cell Surface
Marker
Mature B-Cell & Follicular Dendritic Cell Marker
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CR2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for CD21 Antibody (CR2/3124R)

  • C3DR
  • C3DRSLEB9
  • CD21 antigen
  • CD21
  • Complement C3d receptor
  • complement component (3d/Epstein Barr virus) receptor 2
  • complement receptor type 2
  • CR2
  • EBV receptor
  • Epstein-Barr virus receptor

Background

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP3-07754
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Bioinformatics

Gene Symbol CR2
Uniprot