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Caspase-9 Antibody (LAP6 96-2-22) [Janelia Fluor® 646]

 
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, KD
Clone
LAP6 96-2-22
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB500-209AF350
Alexa Fluor 405
NB500-209AF405
Alexa Fluor 488
NB500-209AF488
Alexa Fluor 532
NB500-209AF532
Alexa Fluor 594
NB500-209AF594
Alexa Fluor 647
NB500-209AF647
Alexa Fluor 700
NB500-209AF700
Alexa Fluor 750
NB500-209AF750
Allophycocyanin
NB500-209APC
Biotin
NB500-209B
DyLight 350
NB500-209UV
DyLight 405
NB500-209V
DyLight 488
NB500-209G
DyLight 550
NB500-209R
DyLight 594
NB500-209DL594
DyLight 650
NB500-209C
DyLight 680
NB500-209FR
DyLight 755
NB500-209IR
FITC
NB500-209F
HRP
NB500-209H
Janelia Fluor 525
NB500-209JF525
Janelia Fluor 549
NB500-209JF549
Janelia Fluor 585
NB500-209JF585
Janelia Fluor 635
NB500-209JF635
Janelia Fluor 669
NB500-209JF669
PE
NB500-209PE
PerCP
NB500-209PCP
Unconjugated
NB500-209
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB500-209
BSA Free
NB500-209SS
BSA Free
NB500-209
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Caspase-9 Antibody (LAP6 96-2-22) [Janelia Fluor® 646] Summary

Immunogen
Recombinant human Caspase 9 pro-domain protein. [UniProt# P55211]
Localization
Cytoplasmic and Mitochondrial
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CASP9
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Caspase-9 Antibody (LAP6 96-2-22) [Janelia Fluor® 646]

  • APAF-3APAF3
  • apoptotic protease activating factor 3
  • Apoptotic protease Mch-6
  • Apoptotic protease-activating factor 3
  • CASP9
  • CASP-9
  • caspase 9, apoptosis-related cysteine peptidase
  • Caspase9
  • Caspase-9
  • EC 3.4.22.62
  • ICE-LAP6CASPASE-9c
  • ICE-like apoptotic protease 6
  • MCH6apoptosis-related cysteine protease

Background

Caspase 9 (CASP9) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. Caspase 9 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 9 form.

There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Caspase 3 is considered an initiator, as initiated caspase-9 goes on to cleave the effector procaspase-3 and procaspase-7.

Caspase 9 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-9 Antibody (NB500-209JF646). (Showing 1 - 1 of 1 FAQ).

  1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
    • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CASP9