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Caspase-7 Antibody (S03-0F2)

Images

 
Western Blot: Caspase-7 Antibody (S03-0F2) [NBP3-14866] - Western blot detection of Caspase-7 in A549, MCF-7, 293T cell lysates using Caspase-7 Rabbit mAb (1:1000 diluted). Predicted band size: 34kDa. Observed band ...read more
Immunohistochemistry-Paraffin: Caspase-7 Antibody (S03-0F2) [NBP3-14866] - Immunohistochemistry of Caspase-7 in paraffin-embedded Human Cholangiocarcinoma using Caspase-7 Rabbit mAb at dilution 1/50

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
S03-0F2
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Caspase-7 Antibody (S03-0F2) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide of human Caspase-7 (Uniprot#: P55210)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CASP7
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1/50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1/1000
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Purity
Affinity purified

Alternate Names for Caspase-7 Antibody (S03-0F2)

  • apoptosis-related cysteine protease
  • Apoptotic protease Mch-3
  • CASP7
  • CASP-7
  • caspase 7, apoptosis-related cysteine peptidase
  • Caspase7
  • Caspase-7
  • EC 3.4.22
  • EC 3.4.22.60
  • ICE-like apoptotic protease 3
  • Lice2 alpha/beta/gamma
  • Mch3

Background

Caspase 7 (CASP7) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. The sequential activation of caspases plays a central role in the execution-phase of cell apoptosis, and Caspase 7 is an executioner protein of apoptosis. It is activated upon cell death stimuli and induces apoptosis.

Caspase 7 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 7 form.

Caspase 7 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-7 Antibody (NBP3-14866) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Caspase-7 Antibody (NBP3-14866) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Caspase-7 Products

Research Areas for Caspase-7 Antibody (NBP3-14866)

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Blogs on Caspase-7.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

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Bioinformatics

Gene Symbol CASP7