![Western Blot: Carbonic Anhydrase I/CA1 Antibody [HRP] [NB600-942] - Western Blot of Goat Carbonic Anhydrase I/CA1/CA1 antibody [HRP]. Lane 1: Opal Prestained Molecular Weight.Lane 2: Carbonic Anhydrase I/CA1 [10ug]. Primary antibody: Anti-Carbonic Anhydrase I/CA1 HRP Antibody at 1:1000 for 1 hr at RT.Secondary antibody: Streptavidin-HRP antibody at 1:40,000 for 30mins at RT.Blocking bufferPredicted/Observed size: ~29kDa.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-[HRP]-Western-Blot-NB600-942-img0001.jpg "Western Blot: Carbonic Anhydrase I/CA1 Antibody [HRP] [NB600-942] - Western Blot of Goat Carbonic Anhydrase I/CA1/CA1 antibody [HRP]. Lane 1: Opal Prestained Molecular Weight.Lane 2: Carbonic Anhydrase I/CA1 [10ug]. Primary antibody: Anti-Carbonic Anhydrase I/CA1 HRP Antibody at 1:1000 for 1 hr at RT.Secondary antibody: Streptavidin-HRP antibody at 1:40,000 for 30mins at RT.Blocking bufferPredicted/Observed size: ~29kDa.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | HRP |
| Concentration | LYOPH |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-77740-0.1mg | |||
| Unconjugated | NB120-6619-0.1mg | |||
| Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. Carbonic Anhydrase I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes ] |
| Immunogen | Carbonic Anhydrase I/CA1/CA1 [Human Erythrocytes] (Uniprot: P00915) |
| Localization | Cytoplasmic |
| Specificity | Cross reactivity against Carbonic Anhydrase I/CA1 from other tissues and species may occur but have not been specifically determined. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | CA1 |
| Purity | Multi-step |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product is tested by ELISA and western blot and useful in immunohistochemistry. Optimal titers should be obtained by researchers. NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly). |
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| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Gentamicin Sulfate |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent) |
Secondary Antibodies |
Isotype Controls |
Research Areas for Carbonic Anhydrase I/CA1 Antibody (NB600-942)Find related products by research area.
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